Document Detail


β-Cell replication and islet neogenesis following partial pancreatectomy.
MedLine Citation:
PMID:  21623169     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Partial pancreatectomy is one of the most commonly used models in the study of β-cell regeneration. The mechanism by which regeneration occurs in this model has been controversial, with some claiming that islet and β-cell neogenesis is important, while others claim that β-cell replication is predominant. Here, we combined a time course analysis with continuous BrdU administration to study β-cell regeneration following partial pancreatectomy. While exocrine cells in regenerating areas were highly proliferative and positive for BrdU, islets in regenerating areas were negative for BrdU one week after partial pancreatectomy, suggesting that they were derived from preexisting islets rather than being neogenic. The insulin-positive cells in ducts that have been reported by others and taken as evidence of β-cell neogenesis were present in regenerating regions of the pancreas, but were relatively uncommon and were not highly proliferative, suggesting that they could not account for significant islet neogenesis. Consistent with a lack of islet neogenesis, regenerating areas following a second partial pancreatectomy were devoid of islets. β-cell replication was detectable at a high frequency two weeks following partial pancreatectomy and was present at a similar frequency in both regenerating and preexisting regions of the pancreas. In summary, our data indicate that islet neogenesis following partial pancreatectomy does not occur.
Authors:
Seung-Hee Lee; Ergeng Hao; Fred Levine
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-07-01
Journal Detail:
Title:  Islets     Volume:  3     ISSN:  1938-2022     ISO Abbreviation:  Islets     Publication Date:    2011 Jul-Aug
Date Detail:
Created Date:  2011-07-04     Completed Date:  2011-11-22     Revised Date:  2014-10-09    
Medline Journal Info:
Nlm Unique ID:  101495366     Medline TA:  Islets     Country:  United States    
Other Details:
Languages:  eng     Pagination:  188-95     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Bromodeoxyuridine / pharmacokinetics
Cell Count
Cell Proliferation*
DNA Replication*
Diabetes Mellitus / physiopathology,  therapy
Disease Models, Animal
Gene Expression Regulation
Immunohistochemistry
Insulin / genetics,  metabolism
Insulin-Secreting Cells / cytology,  physiology*
Islets of Langerhans / cytology,  physiology*
Male
Mice
Mice, Inbred ICR
Pancreatectomy*
RNA, Messenger
Regeneration*
Reverse Transcriptase Polymerase Chain Reaction
Tissue Distribution
Chemical
Reg. No./Substance:
0/Insulin; 0/RNA, Messenger; G34N38R2N1/Bromodeoxyuridine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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