Document Detail


The cell pole: the site of cross talk between the DNA uptake and genetic recombination machinery.
MedLine Citation:
PMID:  23046409     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Natural transformation is a programmed mechanism characterized by binding of free double-stranded (ds) DNA from the environment to the cell pole in rod-shaped bacteria. In Bacillus subtilis some competence proteins, which process the dsDNA and translocate single-stranded (ss) DNA into the cytosol, recruit a set of recombination proteins mainly to one of the cell poles. A subset of single-stranded binding proteins, working as "guardians", protects ssDNA from degradation and limit the RecA recombinase loading. Then, the "mediators" overcome the inhibitory role of guardians, and recruit RecA onto ssDNA. A RecA·ssDNA filament searches for homology on the chromosome and, in a process that is controlled by "modulators", catalyzes strand invasion with the generation of a displacement loop (D-loop). A D-loop resolvase or "resolver" cleaves this intermediate, limited DNA replication restores missing information and a DNA ligase seals the DNA ends. However, if any step fails, the "rescuers" will repair the broken end to rescue chromosomal transformation. If the ssDNA does not share homology with resident DNA, but it contains information for autonomous replication, guardian and mediator proteins catalyze plasmid establishment after inhibition of RecA. DNA replication and ligation reconstitute the molecule (plasmid transformation). In this review, the interacting network that leads to a cross talk between proteins of the uptake and genetic recombination machinery will be placed into prospective.
Authors:
Dawit Kidane; Silvia Ayora; Joann B Sweasy; Peter L Graumann; Juan C Alonso
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Review     Date:  2012-10-09
Journal Detail:
Title:  Critical reviews in biochemistry and molecular biology     Volume:  47     ISSN:  1549-7798     ISO Abbreviation:  Crit. Rev. Biochem. Mol. Biol.     Publication Date:    2012 Nov-Dec
Date Detail:
Created Date:  2012-10-17     Completed Date:  2013-04-19     Revised Date:  2013-11-06    
Medline Journal Info:
Nlm Unique ID:  8903774     Medline TA:  Crit Rev Biochem Mol Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  531-55     Citation Subset:  IM    
Affiliation:
Departments of Therapeutic Radiology and Genetics, Yale University School of Medicine, New Haven, CT, USA.
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MeSH Terms
Descriptor/Qualifier:
Bacillus subtilis / genetics,  metabolism
Bacterial Proteins / genetics,  metabolism
Cytosol / metabolism
DNA / metabolism*
DNA Repair
DNA Replication
DNA Transformation Competence*
DNA, Single-Stranded / metabolism*
DNA-Binding Proteins / genetics,  metabolism
Gene Transfer, Horizontal
Plasmids
Rec A Recombinases / genetics,  metabolism
Recombination, Genetic*
Grant Support
ID/Acronym/Agency:
K01 CA154854/CA/NCI NIH HHS; K01 CA15485401A/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/DNA, Single-Stranded; 0/DNA-Binding Proteins; 9007-49-2/DNA; EC 2.7.7.-/Rec A Recombinases
Comments/Corrections

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