Document Detail

A cell nanoinjector based on carbon nanotubes.
MedLine Citation:
PMID:  17485677     Owner:  NLM     Status:  MEDLINE    
Technologies for introducing molecules into living cells are vital for probing the physical properties and biochemical interactions that govern the cell's behavior. Here, we report the development of a nanoscale cell injection system (termed the nanoinjector) that uses carbon nanotubes to deliver cargo into cells. A single multiwalled carbon nanotube attached to an atomic force microscope (AFM) tip was functionalized with cargo via a disulfide-based linker. Penetration of cell membranes with this "nanoneedle" was controlled by the AFM. The following reductive cleavage of the disulfide bonds within the cell's interior resulted in the release of cargo inside the cells, after which the nanoneedle was retracted by AFM control. The capability of the nanoinjector was demonstrated by injection of protein-coated quantum dots into live human cells. Single-particle tracking was used to characterize the diffusion dynamics of injected quantum dots in the cytosol. This technique causes no discernible membrane or cell damage, and can deliver a discrete number of molecules to the cell's interior without the requirement of a carrier solvent.
Xing Chen; Andras Kis; A Zettl; Carolyn R Bertozzi
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2007-05-07
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  104     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-05-22     Completed Date:  2007-07-02     Revised Date:  2013-06-06    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  8218-22     Citation Subset:  IM    
Department of Chemistry, University of California, Berkeley, CA 94720, USA.
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MeSH Terms
Disulfides / chemistry
HeLa Cells
Microscopy, Atomic Force
Nanotubes, Carbon*
Quantum Dots
Streptavidin / chemistry
Reg. No./Substance:
0/Disulfides; 0/Nanotubes, Carbon; 9013-20-1/Streptavidin

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