Document Detail


A cell-microelectronic sensing technique for the screening of cytoprotective compounds.
MedLine Citation:
PMID:  20198300     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In recent years, a new cell-based high throughput paradigm has emerged, which seeks to identify novel, pharmacologically active cytoprotective compounds. The essence of this approach is to create experimental models of cell injury relevant for a particular disease by establishing in vitro cell-based models, followed by high-throughput testing of compounds that affect the cellular response in a desired manner. Prior approaches typically used simple end-point analyses. To assess the cytoprotective effects of novel drug candidates in real-time, we have applied a cell-microelectronic sensing technique (RT-CES), which measures changes in the impedance of individual microelectronic wells that correlates linearly with cell index (reflecting cell number, adherence and cell growth), thereby allowing the continuous determination of cell viability during oxidative stress. In vitro cytotoxicity was elicited by hydrogen peroxide in myocytes (H9c2) and hepatocytes (Hep3B). Cells were post-treated at 30 min with various reference molecules and novel cytoprotective compounds. Cytoprotection detected in the RT-CES system correlated well with the results of two classical end-point-based methods (improvement in MTT and reduction of LDH release). The RT-CES method, when used as described in the current report, is suitable for the screening of molecular libraries to identify molecules or molecule combinations that attenuate oxidative stress-induced cell damage.
Authors:
B?la Ozsv?ri; L?szl? G Pusk?s; Lajos I Nagy; Iv?n Kanizsai; M?ri? Gyuris; Ram?na Mad?csi; Lili?na Z Feh?r; Domokos Ger?; Csaba Szab?
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of molecular medicine     Volume:  25     ISSN:  1791-244X     ISO Abbreviation:  Int. J. Mol. Med.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-03-03     Completed Date:  2010-05-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9810955     Medline TA:  Int J Mol Med     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  525-30     Citation Subset:  IM    
Affiliation:
Avidin Ltd., Szeged, Szeged, Hungary.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biosensing Techniques / methods*
Cell Line
Cell Survival / drug effects
Cytoprotection / drug effects*
Drug Evaluation, Preclinical / methods*
Electronics / methods*
Microscopy, Fluorescence
Oxidative Stress / drug effects
Rats
Small Molecule Libraries / analysis*,  pharmacology*
Chemical
Reg. No./Substance:
0/Small Molecule Libraries

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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