Document Detail


A cell-free soluble-phase radioimmunoassay for Thy-1 antigen.
MedLine Citation:
PMID:  6140218     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A cell-free, soluble-phase, radioimmunoassay has been developed for Thy-1 antigen. The method is based on immunoprecipitation of radiolabelled Thy-1 molecules with specific antibodies, anti-immunoglobulin serum and polyethyleneglycol (PEG). The method can be used with convenience to screen for the presence of Thy-1 in various fluids as well as on cell surfaces for qualitative or quantitative purposes. Presence of antibodies or autoantibodies against Thy-1 can also be detected specifically. Evidence that the dog, carp, hamster and goldfish carry Thy-1-like molecules on neuronal (brain) cells is demonstrated by this method.
Authors:
A Shalev; F Zuckerman
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Immunology     Volume:  50     ISSN:  0019-2805     ISO Abbreviation:  Immunology     Publication Date:  1983 Dec 
Date Detail:
Created Date:  1984-02-14     Completed Date:  1984-02-14     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0374672     Medline TA:  Immunology     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  667-70     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, Surface / analysis*
Antigens, Thy-1
Brain / immunology
Cattle
Cricetinae
Dogs
Fishes
Humans
Immune Sera / immunology
Mice
Radioimmunoassay / methods
Rats
Reference Values
Chemical
Reg. No./Substance:
0/Antigens, Surface; 0/Antigens, Thy-1; 0/Immune Sera
Comments/Corrections

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