Document Detail

The cell envelope structure and properties of Mycobacterium smegmatis mc(2)155: is there a clue for the unique transformability of the strain?
MedLine Citation:
PMID:  15942014     Owner:  NLM     Status:  MEDLINE    
Mycobacterium smegmatis is often used as a surrogate host for pathogenic mycobacteria, especially since the isolation of the transformable smooth morphotype strain mc(2)155 from the isogenic rough wild-type strain ATCC 607. Biochemical analysis of the cell envelope components revealed a lack of polar glycolipids, namely the lipooligosaccharides and the polar subfamilies of glycopeptidolipids, in the mc(2)155 strain. In addition, the latter strain differs from its parent by the distribution of various species of glycolipids and phospholipids between the outermost and deeper layers of the cell envelope. The presence of filamentous and rope-like structures at the cell surface of mc(2)155 cells grown in complex media further supported an ultrastructural change in the cell envelope of the mutant. Importantly, a significantly more rapid uptake of the hydrophobic chenodeoxycholate was observed for the mutant compared to wild-type cells. Taken together, these data indicate that the nature of the surface-exposed and envelope constituents is crucial for the surface properties, cell wall permeability and bacterial phenotype, and suggest that the transformable character of the mc(2)155 strain may be in part explained by these profound modifications of its cell envelope.
Gilles Etienne; Françoise Laval; Christelle Villeneuve; Premkumar Dinadayala; Ahmed Abouwarda; Didier Zerbib; Alexandra Galamba; Mamadou Daffé
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Microbiology (Reading, England)     Volume:  151     ISSN:  1350-0872     ISO Abbreviation:  Microbiology (Reading, Engl.)     Publication Date:  2005 Jun 
Date Detail:
Created Date:  2005-06-08     Completed Date:  2005-11-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9430468     Medline TA:  Microbiology     Country:  England    
Other Details:
Languages:  eng     Pagination:  2075-86     Citation Subset:  IM    
Institut de Pharmacologie et de Biologie Structurale, Unité Mixte de Recherche du Centre National de la Recherche Scientifique et de l'Université Paul Sabatier, 31077 Toulouse cedex 04, France.
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MeSH Terms
Biological Transport
Cell Membrane / chemistry*
Cell Wall / chemistry*,  ultrastructure
Chenodeoxycholic Acid / metabolism
Chromatography, Thin Layer
Glycolipids / analysis
Glycopeptides / analysis
Lipopolysaccharides / analysis
Microscopy, Electron, Transmission
Mycobacterium smegmatis / chemistry*,  genetics*,  ultrastructure
Phospholipids / analysis
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Reg. No./Substance:
0/Glycolipids; 0/Glycopeptides; 0/Lipopolysaccharides; 0/Phospholipids; 0/lipid-linked oligosaccharides; 474-25-9/Chenodeoxycholic Acid

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