Document Detail

The cell cycle and virus infection.
MedLine Citation:
PMID:  15576934     Owner:  NLM     Status:  MEDLINE    
A number of different viruses interact with the cell cycle in order to subvert host-cell function and increase the efficiency of virus replication; examples can be found from DNA, retro, and RNA viruses. The majority of studies have been conducted on DNA and retroviruses whose primary site of replication is the nucleus, but increasingly a number of researchers are demonstrating that RNA viruses, whose primary site of replication is normally the cytoplasm, also interfere with the cell cycle. Viral interference with the cell cycle can have a myriad of different effects to improve virus infection, for example to promote replication of viral DNA genomes, or to delay the cell cycle to allow sufficient time for RNA virus assembly. Although cell cycle control is fairly well characterized in terms of checkpoints and control molecules (e.g., cyclins), in recent years several researchers have demonstrated that the nucleolus is also involved in cell cycle control. The nucleolus and associated subnuclear structures can sequester cell cycle regulatory complexes, and nucleolar proteins also have a direct and indirect effect on the cycling cell. Viruses also interact with the nucleolus. In order to study the interactions between a virus and the cell cycle and vice versa we have developed and adapted a number of different approaches and strategies. These include determinations of virus yield and measurements of virus replication to flow cytometry and confocal analysis of the host cell. Increasingly we have found that proteomic approaches allow the rapid analysis of a whole plethora of cell cycle proteins that may be affected by virus infection.
Stevan R Emmett; Brian Dove; Laura Mahoney; Torsten Wurm; Julian A Hiscox
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  296     ISSN:  1064-3745     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2005  
Date Detail:
Created Date:  2004-12-03     Completed Date:  2005-03-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  197-218     Citation Subset:  IM    
School of Biochemistry and Microbiology, University of Leeds, Leeds, UK.
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MeSH Terms
Blotting, Northern
Caspase 8
Caspases / analysis
Cell Cycle / physiology*
Cell Nucleolus / virology
Cercopithecus aethiops
Coronavirus / genetics,  pathogenicity,  physiology
Electrophoresis, Gel, Two-Dimensional
Flow Cytometry
Fluorescent Antibody Technique, Indirect
Hela Cells
Nucleocapsid Proteins / genetics
Plaque Assay
Proliferating Cell Nuclear Antigen / analysis
RNA Viruses / pathogenicity*
RNA, Viral / isolation & purification
Vero Cells
Virus Diseases / pathology,  virology
Virus Replication
Reg. No./Substance:
0/Nucleocapsid Proteins; 0/Proliferating Cell Nuclear Antigen; 0/RNA, Viral; 59-14-3/Bromodeoxyuridine; EC 3.4.22.-/CASP8 protein, human; EC 3.4.22.-/Caspase 8; EC 3.4.22.-/Caspases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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