Document Detail


The cell cycle of glial cells grown in vitro: an immunocytochemical method of analysis.
MedLine Citation:
PMID:  1506676     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Studies of cell cycles have traditionally employed [3H]- and [14C]-thymidine to label the DNA of proliferating cells and autoradiography to reveal the thymidine label. The development of antibodies to the thymidine analogue 5-bromodeoxyuridine (BrdU) has allowed the development of an immunocytochemical method analogous to the thymidine autoradiographic technique. In direct comparisons, we found that the immunocytochemical method consistently detected a larger number of proliferating cells. This suggests that it may be a more sensitive index of proliferation than thymidine autoradiography in some systems. We used the BrdU method to analyze the cycle of astroglia cultured from neonatal mouse cerebral cortex. Cells were exposed to BrdU for 1 hr to label a discrete subpopulation of proliferating cells. At 2-36 hr after the pulse, a combination of anti-BrdU immunocytochemistry and counterstaining with propidium iodide was used to identify proliferating cells. The length of the cell cycle was determined by charting the percent of BrdU-labeled mitotic cells vs time after the pulse. We found the average length of the cell cycle of astrocytes grown in vitro to be 20.5 hr. The combined G2 + M phases were 2-3 hr. These values are virtually identical with those found for glial cells in vivo, suggesting that the culture environment does not interfere with the normal control of cell cycle length.
Authors:
P E Knapp
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  40     ISSN:  0022-1554     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  1992 Sep 
Date Detail:
Created Date:  1992-09-21     Completed Date:  1992-09-21     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1405-11     Citation Subset:  IM    
Affiliation:
Department of Anatomy and Cell Biology, Wayne State University, Detroit, Michigan 48201.
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MeSH Terms
Descriptor/Qualifier:
Animals
Astrocytes / cytology,  metabolism
Bromodeoxyuridine
Cell Cycle*
DNA / metabolism
Immunohistochemistry
Mice
Neuroglia / cytology*,  metabolism
Thymidine / metabolism
Grant Support
ID/Acronym/Agency:
NS 15338/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
50-89-5/Thymidine; 59-14-3/Bromodeoxyuridine; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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