| The cell cycle effects of docosahexaenoic acid on human metastatic hepatocellular carcinoma proliferation. | |
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MedLine Citation:
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PMID: 20198345 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Given the reported side effects associated with chemotherapy and surgical resection, dietary intervention with omega-3 polyunsaturated fatty acids (PUFAs) has been postulated to be an alterative way to prevent liver cancer progression and metastasis. We studied the effects of an omega-3 PUFA, docahexaenoic acid (DHA) on COX-2 expression and the cell cycle control machinery that co-ordinately regulate the HCC cells growth. Our data showed that DHA (0-200 microM) retarded proliferation of the human metastatic HCC cell line MHCC97L dose-dependently. In addition, inhibition of cyclin A/Cdk2 interfered with S-phase progression further in agreement with the result of bivariate flow cytometric analysis which indicated that DNA synthesis time (Ts) was significantly prolonged by DHA in MHCC97L. The N-myc oncogene, the heat shock proteins Hsp27 and glucose-related protein 78 (GRP78) as well as the antioxidant enzymes superoxide dismutase may play significant roles in the cell cycle control and reduced-proliferation of MHCC97L by DHA. Our data indicated that it is imperative to develop therapeutic strategy with omega-3 PUFA that simultaneously targets COX-2 and other cell cycle regulators in hepatocarcinogenesis. This study provides novel mechanistic insights into the modulation of DHA on human hepatocarcinoma. |
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Authors:
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Carol Yee-Ki Lee; Wai-Hung Sit; Sheung-Tat Fan; Kwan Man; Irene Wing-Yan Jor; Leo Lap-Yan Wong; Murphy Lam-Yim Wan; Kian Cheng Tan-Un; Jennifer Man-Fan Wan |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: International journal of oncology Volume: 36 ISSN: 1791-2423 ISO Abbreviation: Int. J. Oncol. Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-03-03 Completed Date: 2010-06-04 Revised Date: 2012-06-25 |
Medline Journal Info:
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Nlm Unique ID: 9306042 Medline TA: Int J Oncol Country: Greece |
Other Details:
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Languages: eng Pagination: 991-8 Citation Subset: IM |
Affiliation:
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School of Biological Sciences, The University of Hong Kong, Pokfulam, Hong Kong, P.R. China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antineoplastic Agents
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pharmacology* Carcinoma, Hepatocellular / genetics, metabolism, pathology*, secondary Cell Adhesion / drug effects Cell Cycle / drug effects* Cell Line, Tumor Cell Proliferation / drug effects* Cyclin A / metabolism Cyclin E / metabolism Cyclin-Dependent Kinase 2 / metabolism Cyclooxygenase 2 / genetics, metabolism DNA Replication / drug effects Docosahexaenoic Acids / pharmacology* Dose-Response Relationship, Drug Gene Expression Regulation, Enzymologic / drug effects Gene Expression Regulation, Neoplastic / drug effects HSP27 Heat-Shock Proteins / metabolism Heat-Shock Proteins / metabolism Humans Liver Neoplasms / genetics, metabolism, pathology* Proto-Oncogene Proteins c-myc / metabolism RNA, Messenger / metabolism Superoxide Dismutase / metabolism Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents; 0/Cyclin A; 0/Cyclin E; 0/HSP27 Heat-Shock Proteins; 0/HSPB1 protein, human; 0/Heat-Shock Proteins; 0/Proto-Oncogene Proteins c-myc; 0/RNA, Messenger; 0/molecular chaperone GRP78; 25167-62-8/Docosahexaenoic Acids; EC 1.14.99.1/Cyclooxygenase 2; EC 1.14.99.1/PTGS2 protein, human; EC 1.15.1.1/Superoxide Dismutase; EC 2.7.11.22/CDK2 protein, human; EC 2.7.11.22/Cyclin-Dependent Kinase 2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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