Document Detail


A cell cycle arrest is necessary for bottle cell formation in the early Xenopus gastrula: integrating cell shape change, local mitotic control and mesodermal patterning.
MedLine Citation:
PMID:  16275039     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
During development cell proliferation and morphogenetic movements are tightly intermingled. Both processes depend on the same cytoskeletal elements. Therefore, precise regulation of local mitotic activity seems to be basic for proper embryogenesis. Here, I report on bottle cells as an early non-mitotic cell population in the Xenopus gastrula. Endogenous and activin/BVg1-induced ectopic bottle cells do not proliferate. Overexpression of the mitosis-promoting phosphatase cdc25C increases the proliferation rate and interferes with bottle cell formation whereas the phosphatase-dead mutant cdc25C(C457A) does not. Cdc25C also affects other gastrulation processes such as epiboly, vegetal rotation or tissue separation as inferred from histological inspection of early gastrulae. Double stainings of gsc/Xbra transcripts and mitotic nuclei in ectopic and endogenous lips demonstrated that non-mitotic cells occur in the bottle cell region and, to a lesser extent, in the gsc domain which both are indicative of high TGF-beta signalling. In contrast, the Xbra-region and the remainder of the animal cap appear to be permissive for higher rates of cell proliferation. These data suggest inhibition of cell proliferation by high levels of activin-type signals and a close link of mesodermal and mitotic patterning. Finally, coexpression of eFGF together with activin/BVg1 interferes with TGF-beta-induced bottle cell formation. This inhibitory effect correlates with increased cell proliferation as compared to embryos injected with activin/BVg1 alone. Taken together, these data suggest that TGF-beta and FGF signals play antagonistic roles in bottle cell formation and the spatial control of the cell cycle in early Xenopus gastrulae.
Authors:
Thomas Kurth
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Publication Detail:
Type:  Journal Article     Date:  2005-11-04
Journal Detail:
Title:  Mechanisms of development     Volume:  122     ISSN:  0925-4773     ISO Abbreviation:  Mech. Dev.     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2005-12-12     Completed Date:  2008-08-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9101218     Medline TA:  Mech Dev     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  1251-65     Citation Subset:  IM    
Affiliation:
Fachrichtung Biologie, Studiengang Molekulare Biotechnologie, Fakult?t f?r Mathematik und Naturwissenschaften, Technische Universit?t Dresden, D-01062 Dresden, Germany. thomas.kurth2@mailbox.tu.dresden.de
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MeSH Terms
Descriptor/Qualifier:
Activins / physiology
Animals
Body Patterning / physiology*
Cell Cycle / physiology*
Cell Shape / physiology*
Embryo, Nonmammalian / cytology,  physiology
Fibroblast Growth Factors / physiology
Gastrula / cytology*,  physiology*
Goosecoid Protein / biosynthesis,  genetics
Growth Inhibitors / physiology
Mesoderm / cytology,  physiology*
Mitosis / physiology*
T-Box Domain Proteins / biosynthesis,  genetics
Transforming Growth Factor beta / physiology
Xenopus Proteins / biosynthesis,  genetics
Xenopus laevis
cdc25 Phosphatases / biosynthesis,  genetics
Chemical
Reg. No./Substance:
0/Goosecoid Protein; 0/Growth Inhibitors; 0/T-Box Domain Proteins; 0/Transforming Growth Factor beta; 0/Xbra protein, Xenopus; 0/Xenopus Proteins; 104625-48-1/Activins; 62031-54-3/Fibroblast Growth Factors; EC 3.1.3.48/cdc25 Phosphatases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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