Document Detail


The catabolic enzyme methionine gamma-lyase limits methionine accumulation in potato tubers.
MedLine Citation:
PMID:  24738868     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Increasing methionine in potato tubers is desirable, both to increase the availability of this limiting essential amino acid and to enhance the aroma of baked and fried potatoes. Previous attempts to elevate potato methionine content using transgenic approaches have focused on increasing methionine biosynthesis. Higher isoleucine accumulation in these transgenic tubers suggested that the potatoes compensate for increased methionine biosynthesis with enhanced catabolism via methionine gamma-lyase (MGL), thereby producing 2-ketybutyrate for isoleucine biosynthesis. In the current study, we show that potato StMGL1 encodes a functional MGL in potato tubers. In planta silencing of StMGL1 results in an increased methionine to isoleucine ratio in the free amino acid profile of potato tubers and, in some transgenic lines, elevated accumulation of free methionine. In both wild-type and transgenic tubers, the ratio of methionine to isoleucine is negatively correlated with the level of StMGL1 transcript. A three-dimensional distribution of free amino acids in potato tubers is also described.
Authors:
Tengfang Huang; Vijay Joshi; Georg Jander
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-4-17
Journal Detail:
Title:  Plant biotechnology journal     Volume:  -     ISSN:  1467-7652     ISO Abbreviation:  Plant Biotechnol. J.     Publication Date:  2014 Apr 
Date Detail:
Created Date:  2014-4-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101201889     Medline TA:  Plant Biotechnol J     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2014 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/CK276817.1;  AB061263
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