Document Detail


Cascade of events governing cell-cell fusion induced by herpes simplex virus glycoproteins gD, gH/gL, and gB.
MedLine Citation:
PMID:  20861251     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Herpesviruses minimally require the envelope proteins gB and gH/gL for virus entry and cell-cell fusion; herpes simplex virus (HSV) additionally requires the receptor-binding protein gD. Although gB is a class III fusion protein, gH/gL does not resemble any documented viral fusion protein at a structural level. Based on those data, we proposed that gH/gL does not function as a cofusogen with gB but instead regulates the fusogenic activity of gB. Here, we present data to support that hypothesis. First, receptor-positive B78H1-C10 cells expressing gH/gL fused with receptor-negative B78H1 cells expressing gB and gD (fusion in trans). Second, fusion occurred when gH/gL-expressing C10 cells preexposed to soluble gD were subsequently cocultured with gB-expressing B78 cells. In contrast, prior exposure of gB-expressing C10 cells to soluble gD did not promote subsequent fusion with gH/gL-expressing B78 cells. These data suggest that fusion involves activation of gH/gL by receptor-bound gD. Most importantly, soluble gH/gL triggered a low level of fusion of C10 cells expressing gD and gB; a much higher level was achieved when gB-expressing C10 cells were exposed to a combination of soluble gH/gL and gD. These data clearly show that gB acts as the HSV fusogen following activation by gD and gH/gL. We suggest the following steps leading to fusion: (i) conformational changes to gD upon receptor binding, (ii) alteration of gH/gL by receptor-activated gD, and (iii) upregulation of the fusogenic potential of gB following its interaction with activated gH/gL. The third step may be common to other herpesviruses.
Authors:
Doina Atanasiu; Wan Ting Saw; Gary H Cohen; Roselyn J Eisenberg
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-09-22
Journal Detail:
Title:  Journal of virology     Volume:  84     ISSN:  1098-5514     ISO Abbreviation:  J. Virol.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-11-04     Completed Date:  2010-12-20     Revised Date:  2013-09-19    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  12292-9     Citation Subset:  IM    
Affiliation:
Department of Microbiology, School of Dental Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA. doinaa2@biochem.dental.upenn.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Fusion
Cell Line
Fluorescent Antibody Technique
Giant Cells / cytology
Membrane Fusion / physiology*
Membrane Glycoproteins / metabolism*
Mice
Plasmids / genetics
Simplexvirus / metabolism*
Viral Envelope Proteins / metabolism*
Grant Support
ID/Acronym/Agency:
AI-056045/AI/NIAID NIH HHS; AI-076231/AI/NIAID NIH HHS; AI-18289/AI/NIAID NIH HHS; R01 AI056045/AI/NIAID NIH HHS; R01 AI056045-07/AI/NIAID NIH HHS; R01 AI076231/AI/NIAID NIH HHS; R01 AI076231-15/AI/NIAID NIH HHS; R01 NS036731/NS/NINDS NIH HHS; R21 AI056045/AI/NIAID NIH HHS; R37 AI018289/AI/NIAID NIH HHS; R56 AI076231/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Membrane Glycoproteins; 0/Viral Envelope Proteins
Comments/Corrections

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