| Cascade of events governing cell-cell fusion induced by herpes simplex virus glycoproteins gD, gH/gL, and gB. | |
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MedLine Citation:
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PMID: 20861251 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Herpesviruses minimally require the envelope proteins gB and gH/gL for virus entry and cell-cell fusion; herpes simplex virus (HSV) additionally requires the receptor-binding protein gD. Although gB is a class III fusion protein, gH/gL does not resemble any documented viral fusion protein at a structural level. Based on those data, we proposed that gH/gL does not function as a cofusogen with gB but instead regulates the fusogenic activity of gB. Here, we present data to support that hypothesis. First, receptor-positive B78H1-C10 cells expressing gH/gL fused with receptor-negative B78H1 cells expressing gB and gD (fusion in trans). Second, fusion occurred when gH/gL-expressing C10 cells preexposed to soluble gD were subsequently cocultured with gB-expressing B78 cells. In contrast, prior exposure of gB-expressing C10 cells to soluble gD did not promote subsequent fusion with gH/gL-expressing B78 cells. These data suggest that fusion involves activation of gH/gL by receptor-bound gD. Most importantly, soluble gH/gL triggered a low level of fusion of C10 cells expressing gD and gB; a much higher level was achieved when gB-expressing C10 cells were exposed to a combination of soluble gH/gL and gD. These data clearly show that gB acts as the HSV fusogen following activation by gD and gH/gL. We suggest the following steps leading to fusion: (i) conformational changes to gD upon receptor binding, (ii) alteration of gH/gL by receptor-activated gD, and (iii) upregulation of the fusogenic potential of gB following its interaction with activated gH/gL. The third step may be common to other herpesviruses. |
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Authors:
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Doina Atanasiu; Wan Ting Saw; Gary H Cohen; Roselyn J Eisenberg |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2010-09-22 |
Journal Detail:
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Title: Journal of virology Volume: 84 ISSN: 1098-5514 ISO Abbreviation: J. Virol. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-11-04 Completed Date: 2010-12-20 Revised Date: 2013-04-11 |
Medline Journal Info:
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Nlm Unique ID: 0113724 Medline TA: J Virol Country: United States |
Other Details:
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Languages: eng Pagination: 12292-9 Citation Subset: IM |
Affiliation:
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Department of Microbiology, School of Dental Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA. doinaa2@biochem.dental.upenn.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Fusion Cell Line Fluorescent Antibody Technique Giant Cells / cytology Membrane Fusion / physiology* Membrane Glycoproteins / metabolism* Mice Plasmids / genetics Simplexvirus / metabolism* Viral Envelope Proteins / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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AI-056045/AI/NIAID NIH HHS; AI-076231/AI/NIAID NIH HHS; AI-18289/AI/NIAID NIH HHS; R01 AI056045-07/AI/NIAID NIH HHS; R01 AI076231-15/AI/NIAID NIH HHS; R37 AI018289/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Membrane Glycoproteins; 0/Viral Envelope Proteins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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