Document Detail

A carmine-Giemsa staining technic for meiotic prophase chromosomes of the genus Beta L.
MedLine Citation:
PMID:  83036     Owner:  NLM     Status:  MEDLINE    
For a detailed study of chromosome morphology in meiotic prophase stages of Beta species, a special double staining technic has been developed. It consists of combined maceration-staining in an ethanol-hydrochloric acid-carmine mixture followed by poststaining of the squashed material in a diluted Giemsa solution. The technic yields well-spread prophase meiotic nuclei showing detailed structures both in weaker stained chromosome segments and in threadlike chromatin structures. This technic proved to be especially favorable for stages which are difficult to interpret, such as pachytene, schizotene and diffuse stages.
J H de Jong
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Stain technology     Volume:  53     ISSN:  0038-9153     ISO Abbreviation:  Stain Technol     Publication Date:  1978 May 
Date Detail:
Created Date:  1979-02-26     Completed Date:  1979-02-26     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0404535     Medline TA:  Stain Technol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  169-72     Citation Subset:  IM    
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MeSH Terms
Azure Stains / diagnostic use
Chromosomes / ultrastructure*
Plants / ultrastructure*
Staining and Labeling*
Reg. No./Substance:
0/Azure Stains

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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