Document Detail


A carboxy-terminal mutant spleen focus-forming virus (SFFV) envelope glycoprotein is transport-competent, but non-leukemogenic.
MedLine Citation:
PMID:  1441737     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The Friend spleen focus-forming virus (F-SFFV) codes for a transport-defective leukemogenic envelope glycoprotein designated as gp52. We have previously shown that the external domain of gp52 carries the determinants responsible for its transport defect. Consistent with this idea, truncated gp52 molecules that lack a hydrophobic membrane anchor were transport-defective, and were not secreted from cells. In this report, we describe the construction of a mutant SFFV envelope gene that codes for altered gp52 molecules in which the carboxyl-terminal hydrophobic residues are replaced with exogenous hydrophilic residues encoded by the vector-derived sequences. The mutant env gene was expressed using the retroviral expression vector, pLXSN, and the mutant envelope protein was found to be transport-competent, and efficiently secreted from the cells. However, M-MuLV pseudotypes of the retroviral vectors expressing the mutant genome were found to be non-leukemogenic in mice.
Authors:
R V Srinivas; Z Rui; R W Compans
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Virus research     Volume:  26     ISSN:  0168-1702     ISO Abbreviation:  Virus Res.     Publication Date:  1992 Oct 
Date Detail:
Created Date:  1992-12-04     Completed Date:  1992-12-04     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8410979     Medline TA:  Virus Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  57-69     Citation Subset:  IM; X    
Affiliation:
Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, TN 38101.
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MeSH Terms
Descriptor/Qualifier:
3T3 Cells
Amino Acid Sequence
Animals
Base Sequence
Biological Transport
Cell Line
Genes, env*
Leukemia, Erythroblastic, Acute / genetics,  microbiology*
Mice
Molecular Sequence Data
Molecular Weight
Moloney murine leukemia virus / genetics
Mutation
Spleen Focus-Forming Viruses / genetics*
Viral Envelope Proteins / chemistry,  classification,  genetics,  metabolism*
Grant Support
ID/Acronym/Agency:
CA18611/CA/NCI NIH HHS; CA40440/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Viral Envelope Proteins; 0/gp52 protein, spleen focus-forming virus

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