Document Detail

The carbon dioxide production rate assumption biases gastric emptying parameters in healthy adults.
MedLine Citation:
PMID:  23322660     Owner:  NLM     Status:  In-Data-Review    
RATIONALE: An altered gastric emptying (GE) rate has been implicated in the aetiology of obesity. The (13) C-octanoic acid breath test (OBT) is frequently used to measure GE, and the cumulative percentage of (13) C recovered (cPDR) is a common outcome measure. However, true cPDR in breath is dependent on accurate measurement of carbon dioxide production rate (V˙CO2). The current study aimed to quantify differences in the (13) C OBT results obtained using directly measured V˙CO2 (V˙CO2DM) compared with (i) predicted from resting V˙CO2 (V˙CO2PR) and (ii) predicted from body surface area V˙CO2 (V˙CO2BSA).
METHODS: The GE rate of a high-fat test meal was assessed in 27 lean subjects using the OBT. Breath samples were gathered during the fasted state and at regular intervals throughout the 6-h postprandial period for determination of (13) C-isotopic enrichment by continuous-flow isotope-ratio mass spectrometry. The V˙CO2 was measured directly from exhaled air samples and the PDR calculated by three methods. The bias and the limits of agreement were calculated using Bland-Altman plots.
RESULTS: Compared with the V˙CO2DM, the cPDR was underestimated by V˙CO2PR (4.8%; p = 0.0001) and V˙CO2BSA (2.7%; p = 0.02). The GE T(half) was underestimated by V˙CO2PR (13 min; p = 0.0001) and V˙CO2BSA (10 min; p = 0.01), compared with V˙CO2DM.
CONCLUSIONS: The findings highlight the importance of directly measuring V˙CO2 production rates throughout the (13) C OBT and could partly explain the conflicting evidence regarding the effect of obesity on GE rates. Copyright © 2013 John Wiley & Sons, Ltd.
Oonagh Markey; Amir Shafat
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Rapid communications in mass spectrometry : RCM     Volume:  27     ISSN:  1097-0231     ISO Abbreviation:  Rapid Commun. Mass Spectrom.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-01-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8802365     Medline TA:  Rapid Commun Mass Spectrom     Country:  England    
Other Details:
Languages:  eng     Pagination:  539-45     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 John Wiley & Sons, Ltd.
Hugh Sinclair Unit of Human Nutrition and Institute for Cardiovascular and Metabolic Research (ICMR), Department of Food and Nutritional Sciences, University of Reading, Whiteknights Campus, PO Box 266, Reading, RG6 6AP, UK; Department of Physical Education and Sport Sciences, University of Limerick, Castletroy, Limerick, Ireland.
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