Document Detail


The carbohydrate content of IgG from patients with cystic fibrosis.
MedLine Citation:
PMID:  6657324     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We compare the complete carbohydrate composition of IgG purified from the serum of patients with cystic fibrosis (CF) with that of control subjects. Our results indicate that IgG from cystic fibrosis patients is underglycosylated with respect to galactose and sialic acid, the two terminal sugars on the antennae of the complex-type oligosaccharide chains found on IgG. The galactose content, as determined by gas liquid chromatography, was 2.81 +/- 0.86 (S.D.) mole/mole of IgG for normal subjects versus 1.5 +/- 0.39 for CF subjects (P less than 0.025). Sialic acid content, as determined by the Warren procedure, was 1.33 +/- 0.39 for normals versus 0.47 +/- 0.10 for CF subjects (P less than 0.001). Neither galactose nor sialic acid values for the two groups overlapped. The contents of the core sugars, mannose and glucosamine, and of fucose were not significantly different. When the data are expressed as residues per 3 moles mannose, similar results are obtained. We suggest that immune complex formation, which has been documented in many CF patients, exposes sugar chains of IgG molecules to hydrolytic activity of serum glucosidases, resulting in partial removal of the more peripheral sugars. Because serum glycoproteins missing sialic acid and galactose are not readily cleared from the circulation, the observed changes may contribute to elevated levels of IgG and immune complexes in older people with CF.
Authors:
R Margolies; T F Boat
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Pediatric research     Volume:  17     ISSN:  0031-3998     ISO Abbreviation:  Pediatr. Res.     Publication Date:  1983 Dec 
Date Detail:
Created Date:  1984-02-14     Completed Date:  1984-02-14     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0100714     Medline TA:  Pediatr Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  931-5     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Carbohydrates / analysis*
Child, Preschool
Chromatography, Gas
Cystic Fibrosis / metabolism*
Electrophoresis, Polyacrylamide Gel
Fucose / analysis
Galactose / analysis
Glucosamine / analysis
Humans
Immunoglobulin G / analysis*
Mannose / analysis
Sialic Acids / analysis
Grant Support
ID/Acronym/Agency:
AM 08305/AM/NIADDK NIH HHS
Chemical
Reg. No./Substance:
0/Carbohydrates; 0/Immunoglobulin G; 0/Sialic Acids; 26566-61-0/Galactose; 31103-86-3/Mannose; 3416-24-8/Glucosamine; 3713-31-3/Fucose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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