Document Detail


The carbohydrate of bovine prothrombin. Occurrence of Gal beta 1 leads to 3GlcNAc grouping in asparagine-linked sugar chains.
MedLine Citation:
PMID:  447725     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Bovine prothrombin contains three asparagine-linked sugar chains in 1 molecule. The sugar chains were quantitatively released from the polypeptide backbone by hydrazinolysis. All of the oligosaccharides thus obtained contain N-acetylneuraminic acid. Sialidase treatment of these acidic oligosaccharides released three isomeric oligosaccharides, N-1, N-2 and N-3. N-3 was a typical complex type asparagine-linked sugar chain widely found in other glycoprotein, while N-1 and N-2 were unique, because they contain Gal beta 1 leads to 3GlcNAc grouping in the outer chain moiety. By comparing the data of methylation analysis of the acidic oligosaccharides before and after sialidase treatment, the structures of the sugar chains of bovine prothrombin were confirmed as a mixture of NeuAc alpha 2 leads to 6Gal beta 1 leads to 4GlcNAc beta 1 leads to 2Man alpha 1 leads to 6(NeuAc alpha 2 leads to 6Gal beta 1 leads to 4GlcNAc beta 1 leads to 2Man alpha 1 leads to 3)Man beta 1 leads to 4GlcNAc beta 1 leads to 4GlcNAc leads to Asn, NeuAc alpha 2 leads to 6Gal beta 1 leads to 4GlcNAc beta 1 leads to 2Man alpha 1 leads to 6[NeuAc alpha 2 leads to 3Gal beta 1 leads to 3(NeuAc alpha 2 leads to 6)GlcNAc beta 1 leads to 2Man alpha 1 leads to 3]Man beta 1 leads to 4GlcNAc beta 1 leads to 4GlcNAc leads to Asn, NeuAc alpha 2 leads to 3Gal beta 1 leads to 3(NeuAc alpha 2 leads to 6)GlcNAc beta 1 leads to 2Man alpha 1 leads to 6[NeuAc alpha 2 leads to 3Gal beta 1 leads to 3(NeuAc alpha 2 leads to 6)GlcNAc beta 1 leads to 2Man alpha 1 leads to 3]Man beta 1 leads to 4GlcNAc beta 1 leads to 4GlcNAc leads to Asn and their partially desialized forms.
Authors:
T Mizuochi; K Yamashita; K Fujikawa; W Kisiel; A Kobata
Related Documents :
11749955 - Tailoring the substrate specificity of the beta-glycosidase from the thermophilic archa...
3780745 - Structural analysis of the asparagine-linked oligosaccharides of rat haptoglobin metabo...
10913295 - Recognition of chitooligosaccharides and their n-acetyl groups by putative subsites of ...
21524995 - Cytosolic cd38 protein forms intact disulfides and is active in elevating intracellular...
11996095 - Further studies on the role of phospholipids in determining the characteristics of mito...
122975 - Partial resolution of the enzymes catalyzing photophosphorylation. xv. approaches to th...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  254     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1979 Jul 
Date Detail:
Created Date:  1979-09-01     Completed Date:  1979-09-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  6419-25     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Acetylglucosamine / analysis*
Animals
Asparagine
Carbohydrates / analysis
Cattle
Galactose / analysis*
Glucosamine / analogs & derivatives*
Methylation
Molecular Conformation
Prothrombin*
Chemical
Reg. No./Substance:
0/Carbohydrates; 26566-61-0/Galactose; 3416-24-8/Glucosamine; 7006-34-0/Asparagine; 7512-17-6/Acetylglucosamine; 9001-26-7/Prothrombin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Structure of the carbohydrate moieties of bovine rhodopsin.
Next Document:  Stereochemistry and mechanism of reactions catalyzed by indolyl-3-alkane alpha-hydroxylase.