Document Detail


A capture enzyme-linked immunosorbent assay for virus infectivity titrations as exemplified in an adenovirus system.
MedLine Citation:
PMID:  8315025     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An enzyme-linked immunosorbent assay (ELISA), employing a capturing antihexon monoclonal antibody specifically recognizing free hexons, was developed for quantitative infectivity titration of adenovirus in a microscale titration assay. The method is based on the quantitative assessment of the total excess production of the major structural protein late in infection in samples consisting of 10(5) virus-infected HeLa cells maintained as stationary suspension cultures. Results are obtained with a coefficient of variation of 10% within 50 hours after virus infection. The method was designed for monitoring substances interfering with viral replication, e.g., neutralizing antibodies or antiviral drugs. Since it measured the total antigen content associated with cells as well as antigens possibly released into the growth medium the general approach should be applicable to any viral system where a structural protein is synthesized in excess.
Authors:
E Everitt; M J Varga
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of immunoassay     Volume:  14     ISSN:  0197-1522     ISO Abbreviation:  J Immunoassay     Publication Date:    1993 Mar-Jun
Date Detail:
Created Date:  1993-07-28     Completed Date:  1993-07-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8007167     Medline TA:  J Immunoassay     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1-19     Citation Subset:  IM    
Affiliation:
Department of Microbiology, University of Lund, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Adenoviridae / immunology,  isolation & purification,  physiology
Antibodies, Monoclonal / immunology
Antibodies, Viral / immunology
Capsid / analysis
Capsid Proteins*
Enzyme-Linked Immunosorbent Assay*
Hela Cells / microbiology
Humans
Viral Proteins / analysis*
Virology / methods*
Virus Replication
Viruses / isolation & purification*
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antibodies, Viral; 0/Capsid Proteins; 0/Viral Proteins; 0/hexon capsid protein, Adenovirus

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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