Document Detail

The capsular polysaccharide from Klebsiella serotype K54; location of the O-acyl groups, and a revised structure.
MedLine Citation:
PMID:  7116359     Owner:  NLM     Status:  MEDLINE    
The capsular polysaccharide from Klebsiella type K54, containing both O-formyl and O-acetyl groups, has been investigated by using the techniques of methylation analysis (by gas-liquid chromatography), periodate oxidation-Smith degradation, and both 1H- and 13C-n.m.r. spectroscopy. Degradation of the native polysaccharide with a bacteriophage-induced glucosidase generated a formylated, as well as a formylated and acetylated, tetrasaccharide, whereas similar depolymerization of the deacetylated polysaccharide yielded a single tetrasaccharide; the corresponding, O-acylated octasaccharides were also isolated and characterized. These oligosaccharides, utilized in chemical and spectroscopic studies in order to determine the location of the O-acyl substituents in the repeating sequence, indicated formylation at 0-4 of each lateral D-glucosyl group and acetylation at 0-2 of alternate L-fucosyl residues. A new structure for the repeating unit in the polysaccharide is proposed.
G G Dutton; E H Merrifield
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Carbohydrate research     Volume:  105     ISSN:  0008-6215     ISO Abbreviation:  Carbohydr. Res.     Publication Date:  1982 Jul 
Date Detail:
Created Date:  1982-12-02     Completed Date:  1982-12-02     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0043535     Medline TA:  Carbohydr Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  189-203     Citation Subset:  IM    
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MeSH Terms
Carbohydrate Conformation
Carbohydrate Sequence
Chemical Phenomena
Klebsiella / immunology*
Magnetic Resonance Spectroscopy
Polysaccharides, Bacterial / immunology*
Reg. No./Substance:
0/Polysaccharides, Bacterial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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