| The capsular polysaccharide from Klebsiella serotype K54; location of the O-acyl groups, and a revised structure. | |
MedLine Citation:
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PMID: 7116359 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The capsular polysaccharide from Klebsiella type K54, containing both O-formyl and O-acetyl groups, has been investigated by using the techniques of methylation analysis (by gas-liquid chromatography), periodate oxidation-Smith degradation, and both 1H- and 13C-n.m.r. spectroscopy. Degradation of the native polysaccharide with a bacteriophage-induced glucosidase generated a formylated, as well as a formylated and acetylated, tetrasaccharide, whereas similar depolymerization of the deacetylated polysaccharide yielded a single tetrasaccharide; the corresponding, O-acylated octasaccharides were also isolated and characterized. These oligosaccharides, utilized in chemical and spectroscopic studies in order to determine the location of the O-acyl substituents in the repeating sequence, indicated formylation at 0-4 of each lateral D-glucosyl group and acetylation at 0-2 of alternate L-fucosyl residues. A new structure for the repeating unit in the polysaccharide is proposed. |
Authors:
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G G Dutton; E H Merrifield |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Carbohydrate research Volume: 105 ISSN: 0008-6215 ISO Abbreviation: Carbohydr. Res. Publication Date: 1982 Jul |
Date Detail:
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Created Date: 1982-12-02 Completed Date: 1982-12-02 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0043535 Medline TA: Carbohydr Res Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 189-203 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Carbohydrate Conformation Carbohydrate Sequence Chemical Phenomena Chemistry Klebsiella / immunology* Magnetic Resonance Spectroscopy Polysaccharides, Bacterial / immunology* Serotyping |
| Chemical | |
Reg. No./Substance:
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0/Polysaccharides, Bacterial |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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