Document Detail


The capacity of foodstuffs to induce innate immune activation of human monocytes in vitro is dependent on food content of stimulants of Toll-like receptors 2 and 4.
MedLine Citation:
PMID:  20849668     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
The ingestion of fatty meals is associated with a transient, low-grade systemic inflammatory response in human subjects, involving the activation of circulating monocytes and the secretion of pro-inflammatory cytokines. However, it is not yet clear how different foodstuffs may promote inflammatory signalling. In a screen of forty filter-sterilised soluble extracts from common foodstuffs, seven were found to induce the secretion of TNF-α and IL-6 from human monocytes in vitro. To investigate what may differentiate inflammatory from non-inflammatory food extracts, stimulants of Toll-like receptor (TLR) 2 and TLR4 were quantified using human embryonic kidney-293 cells transfected with each TLR, and calibrated with defined bacterial lipopeptide (BLP) and lipopolysaccharide (LPS) standards. These assays revealed that while most foods contained undetectable levels of TLR2 or TLR4 stimulants, all TNF-α-inducing foods contained stimulants of either TLR2 (up to 1100 ng BLP-equivalent/g) or TLR4 (up to 2700 ng LPS-equivalent/g) in both the soluble and insoluble fractions. TLR stimulants were present mainly in meat products and processed foods, but were minimal or undetectable in fresh fruit and vegetables. The capacity of food extracts to induce TNF-α secretion in monocytes correlated with the content of both TLR2 (r 0·837) and TLR4 stimulants (r 0·748), and was completely abolished by specific inhibition of TLR2 and TLR4. LPS and BLP were found to be highly resistant to typical cooking times and temperatures, low pH and protease treatment. In conclusion, apparently unspoiled foodstuffs can contain large quantities of stimulants of TLR2 and TLR4, both of which may regulate their capacity to stimulate inflammatory signalling.
Authors:
Clett Erridge
Related Documents :
2841048 - Epstein-barr virus activation in raji cells by extracts of preserved food from high ris...
11600018 - Interspecific variation in anthocyanins, phenolics, and antioxidant capacity among geno...
19585468 - Cytochrome p450 2c19 inhibitory activity of common berry constituents.
4309188 - Isolation and identification of phospholipids of bovine rhodopsin.
20410248 - Stress, food, and inflammation: psychoneuroimmunology and nutrition at the cutting edge.
20958668 - Agriculture and food security in ethiopia.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-09-20
Journal Detail:
Title:  The British journal of nutrition     Volume:  105     ISSN:  1475-2662     ISO Abbreviation:  Br. J. Nutr.     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-01-05     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372547     Medline TA:  Br J Nutr     Country:  England    
Other Details:
Languages:  eng     Pagination:  15-23     Citation Subset:  IM    
Affiliation:
Department of Cardiovascular Sciences, University of Leicester, Glenfield General Hospital, Groby Road, Leicester LE3 9QP, UK. ce55@le.ac.uk
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Diversity of sponge mitochondrial introns revealed by cox 1 sequences of Tetillidae.
Next Document:  The prevalence and abundance of helminth parasites in stray dogs from the city of Queretaro in centr...