Document Detail


The capability of reprogramming the male chromatin after fertilization is dependent on the quality of oocyte maturation.
MedLine Citation:
PMID:  15985629     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The present experiments compared the ability of pig oocytes matured either in vivo or in vitro to structurally reorganize the penetrated sperm chromatin into male pronucleus (PN) and to carry out, in parallel, the epigenetic processes of global chromatin methylation and acetylation, 12-14 h after in vitro fertilization (IVF). In addition, PN distribution of histone deacetylase (HDAC), a major enzyme interfacing DNA methylation and histone acetylation, was investigated. The ability of the oocyte to operate an efficient block to polyspermy was markedly affected by maturation. The monospermic fertilization rate was significantly higher for in vivo than for in vitro matured (IVM) oocytes (P < 0.01) which, furthermore, showed a reduced ability to transform the chromatin of penetrated sperm into male PN (P < 0.01). Indirect immunofluorescence analysis of global DNA methylation, histone acetylation and HDAC distribution (HDAC-1, -2 and -3), carried out in monospermic zygotes that reached the late PN stage, showed that IVM oocytes also had a reduced epigenetic competence. In fact, while in about 80% of in vivo matured and IVF oocytes the male PN underwent a process of active demethylation and showed a condition of histone H4 hyperacetylation, only 40% of IVM/IVF zygotes displayed a similar PN remodelling asymmetry. Oocytes that carried out the first part of maturation in vivo (up to germinal vesicle breakdown; GVBD) and then completed the process in vitro, displayed the same PN asymmetry as oocytes matured entirely in vivo. A crucial role of HDAC in the establishment of PN acetylation asymmetry seems to be confirmed by the use of HDAC inhibitors as well as by the abnormal distribution of the enzyme between the two PN in IVM zygotes. Collectively, these data demonstrated that some pig IVM oocytes fail to acquire full remodelling competence which is independent from their ooplasmic ability to morphologically reorganize the sperm nucleus into PN.
Authors:
Luisa Gioia; Barbara Barboni; Maura Turriani; Giulia Capacchietti; Maria Gabriella Pistilli; Paolo Berardinelli; Mauro Mattioli
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Reproduction (Cambridge, England)     Volume:  130     ISSN:  1470-1626     ISO Abbreviation:  Reproduction     Publication Date:  2005 Jul 
Date Detail:
Created Date:  2005-06-29     Completed Date:  2005-12-28     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  100966036     Medline TA:  Reproduction     Country:  England    
Other Details:
Languages:  eng     Pagination:  29-39     Citation Subset:  IM    
Affiliation:
Dipartimento di Scienze Biomediche Comparate, Fisiologia Veterinaria, Università di Teramo, Piazza Aldo Moro 45, Teramo 64100, Italy. lgoia@unite.it
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MeSH Terms
Descriptor/Qualifier:
Acetylation
Animals
Chromatin Assembly and Disassembly*
DNA Methylation
Female
Fertilization in Vitro / methods
Fluorescent Antibody Technique
Histone Deacetylases / metabolism
Histones
Male
Microscopy, Confocal
Oogenesis / physiology*
Pregnancy
Sperm-Ovum Interactions / physiology*
Swine
Time Factors
Zygote / ultrastructure*
Zygote Intrafallopian Transfer
Chemical
Reg. No./Substance:
0/Histones; EC 3.5.1.98/Histone Deacetylases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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