| The canonical Wnt pathway shapes niches supportive of hematopoietic stem/progenitor cells. | |
| | |
MedLine Citation:
|
PMID: 22117039 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Considerable information has accumulated about components of BM that regulate the survival, self-renewal, and differentiation of hematopoietic cells. In the present study, we investigated Wnt signaling and assessed its influence on human and murine hematopoiesis. Hematopoietic stem/progenitor cells (HSPCs) were placed on Wnt3a-transduced OP9 stromal cells. The proliferation and production of B cells, natural killer cells, and plasmacytoid dendritic cells were blocked. In addition, some HSPC characteristics were maintained or re-acquired along with different lineage generation potentials. These responses did not result from direct effects of Wnt3a on HSPCs, but also required alterations in the OP9 cells. Microarray, PCR, and flow cytometric experiments revealed that OP9 cells acquired osteoblastic characteristics while down-regulating some features associated with mesenchymal stem cells, including the expression of angiopoietin 1, the c-Kit ligand, and VCAM-1. In contrast, the production of decorin, tenascins, and fibromodulin markedly increased. We found that at least 1 of these extracellular matrix components, decorin, is a regulator of hematopoiesis: upon addition of this proteoglycan to OP9 cocultures, decorin caused changes similar to those caused by Wnt3a. Furthermore, hematopoietic stem cell numbers in the BM and spleen were elevated in decorin-knockout mice. These findings define one mechanism through which canonical Wnt signaling could shape niches supportive of hematopoiesis. |
| | |
Authors:
|
Michiko Ichii; Mark Barton Frank; Renato V Iozzo; Paul W Kincade |
Related Documents
:
|
21835229 - Cytotoxicity of chemical constituents from the stems of dalbergia parviflora. 22078809 - Recovery of cardiac function mediated by msc and interleukin-10 plasmid functionalised ... 22122049 - Epigenetic regulation of telomere chromatin integrity in pluripotent embryonic stem cells. 21944669 - Administering human adipose-derived mesenchymal stem cells to prevent and treat experim... 21692039 - Ovarian pluripotent/multipotent stem cells and in vitro oogenesis in mammals. 21861689 - Reduced oxygen concentration enhances conversion of embryonic stem cells to epiblast st... 2013699 - Generation of stimulated, lymphokine activated t killer (t-lak) cells from the peripher... 19171879 - Il-17-producing human peripheral regulatory t cells retain suppressive function. 160889 - Comparison of natural killer cells induced by kunjin virus and corynebacterium parvum w... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural Date: 2011-11-23 |
Journal Detail:
|
Title: Blood Volume: 119 ISSN: 1528-0020 ISO Abbreviation: Blood Publication Date: 2012 Feb |
Date Detail:
|
Created Date: 2012-02-20 Completed Date: 2012-05-01 Revised Date: 2013-04-12 |
Medline Journal Info:
|
Nlm Unique ID: 7603509 Medline TA: Blood Country: United States |
Other Details:
|
Languages: eng Pagination: 1683-92 Citation Subset: AIM; IM |
Affiliation:
|
Immunobiology and Cancer Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Animals Cell Differentiation / genetics Cell Proliferation Cells, Cultured Gene Expression Profiling Hematopoiesis / genetics Hematopoietic Stem Cells / cytology*, metabolism, physiology* Humans Infant, Newborn Lymphopoiesis / genetics Mice Mice, Inbred C57BL Mice, Knockout Microarray Analysis Stem Cell Niche / genetics, physiology* Wnt Signaling Pathway / genetics, physiology* |
| Grant Support | |
ID/Acronym/Agency:
|
AI020069/AI/NIAID NIH HHS; CA39481/CA/NCI NIH HHS; R01 CA039481/CA/NCI NIH HHS |
| Comments/Corrections | |
Comment In:
|
Blood. 2012 Feb 16;119(7):1618-9
[PMID:
22343661
]
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A retrospective multicenter analysis of elderly Hodgkin lymphoma: outcomes and prognostic factors in...
Next Document: A novel lentivector targets gene transfer into hHSC in marrow from patients with BM-failure-syndrome...