Document Detail


The canine spleen in oxygen transport: gas exchange and hemodynamic responses to splenectomy.
MedLine Citation:
PMID:  17673565     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In athletic animals the spleen induces acute polycythemia by dynamic contraction that releases red blood cells into the circulation in response to increased O(2) demand and metabolic stress; when energy demand is relieved, the polycythemia is rapidly reversed by splenic relaxation. We have shown in adult foxhounds that splenectomy eliminates exercise-induced polycythemia, thereby reducing peak O(2) uptake and lung diffusing capacity for carbon monoxide (DL(CO)) as well as exaggerating preexisting DL(CO) impairment imposed by pneumonectomy (Dane DM, Hsia CC, Wu EY, Hogg RT, Hogg DC, Estrera AS, Johnson RL Jr. J Appl Physiol 101: 289-297, 2006). To examine whether the postsplenectomy reduction in DL(CO) leads to abnormalities in O(2) diffusion, ventilation-perfusion inequality, or hemodynamic function, we studied these animals via the multiple inert gas elimination technique at rest and during exercise at a constant workload equivalent to 50% or 80% of peak O(2) uptake while breathing 21% and 14% O(2) in balanced order. From rest to exercise after splenectomy, minute ventilation was significantly elevated with respect to O(2) uptake compared with exercise before splenectomy; cardiac output, O(2) delivery, and mean pulmonary and systemic arterial blood pressures were 10-20% lower, while O(2) extraction was elevated with respect to O(2) uptake. Ventilation-perfusion inequality was unchanged, but O(2) diffusing capacities of lung (DL(O2)) and peripheral tissue during exercise were lower with respect to cardiac output postsplenectomy by 32% and 25%, respectively. The relationship between DL(O2) and DL(CO) was unchanged by splenectomy. We conclude that the canine spleen regulates both convective and diffusive O(2) transport during exercise to increase maximal O(2) uptake.
Authors:
Connie C W Hsia; Robert L Johnson; D Merrill Dane; Eugene Y Wu; Aaron S Estrera; Harrieth E Wagner; Peter D Wagner
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2007-08-02
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  103     ISSN:  8750-7587     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  2007 Nov 
Date Detail:
Created Date:  2007-10-29     Completed Date:  2008-01-08     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1496-505     Citation Subset:  IM    
Affiliation:
Dept. of Internal Medicine, Univ. of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75390-9034, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Pressure
Blood Volume
Carbon Dioxide / metabolism
Carbon Monoxide / metabolism
Cardiac Output
Diffusion
Dogs
Erythrocyte Volume
Hematocrit
Hemodynamics*
Hemoglobins / metabolism
Hydrogen-Ion Concentration
Lung / metabolism*
Male
Oxygen / blood,  metabolism*
Oxygen Consumption
Physical Exertion / physiology*
Pulmonary Diffusing Capacity
Pulmonary Gas Exchange*
Spleen / physiology*,  surgery
Splenectomy*
Vascular Resistance
Grant Support
ID/Acronym/Agency:
HL040070/HL/NHLBI NIH HHS; HL054060/HL/NHLBI NIH HHS; HL062873/HL/NHLBI NIH HHS; R01 HL045716/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Hemoglobins; 124-38-9/Carbon Dioxide; 630-08-0/Carbon Monoxide; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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