Document Detail

A canine portal hypertension model induced by intra-portal administration of Sephadex microsphere.
MedLine Citation:
PMID:  20492334     Owner:  NLM     Status:  MEDLINE    
BACKGROUND AND AIM: Big animal models of portal hypertension are important for the research into this disease. The aim of this study was to establish a canine portal hypertension model by intra-portal administration of microspheres. METHODS: Sixteen mongrel dogs were assigned to control group and experimental group randomly. The catheterization of portal vein was performed by laparotomy and the outer end of the catheter was fixed subcutaneously in the abdominal wall. The dogs of the experimental group were given intra-portal injections of microspheres at a five-day interval, six times in total. Portal hemodynamics, blood cell counting, liver and renal function test, portography, gastroscopy, liver, spleen and lung histological examination were taken to evaluate the model. RESULTS: 1, 2, 3 and 4 months after initial injection of microspheres, portal venous pressure rose from baseline 8.7 +/- 0.7 mmHg to 24.3 +/- 1.6, 20.6 +/- 2.1, 19.0 +/- 1.8 and 17.7 +/- 2.0 mmHg, respectively (P < 0.01). The diameter of portal vein increased from 7.6 +/- 0.3 to 8.6 +/- 0.3 mm, calculated portal resistance increased from 0.46 +/- 0.06 to 1.06 +/- 0.20 (mmHg/mL/min/kg body weight); velocity of portal blood flow decreased from 35.1 +/- 1.7 to 26.1 +/- 2.4 cm/s (P < 0.01, respectively). The animals of experimental group developed marked splenomegaly and profuse portosystemic collateral circulations with normal liver and renal function. CONCLUSION: Repeated intra-portal administration of microspheres can induce stable and reproducible chronic portal hypertension in dogs with normal liver and renal functions. This model can meet multiple demands of both basic and clinical research of portal hypertension.
Wangxun Jin; Liming Deng; Qiyu Zhang; Dexin Lin; Jiying Zhu; Yunzhi Chen; Bicheng Chen; Jianmin Li
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of gastroenterology and hepatology     Volume:  25     ISSN:  1440-1746     ISO Abbreviation:  J. Gastroenterol. Hepatol.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-05-24     Completed Date:  2010-08-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8607909     Medline TA:  J Gastroenterol Hepatol     Country:  Australia    
Other Details:
Languages:  eng     Pagination:  778-85     Citation Subset:  IM    
First Affiliated Hospital of Wenzhou Medical College, General Surgery, Wenzhou City, Zhejiang, China.
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MeSH Terms
Angiography, Digital Subtraction
Blood Flow Velocity
Chronic Disease
Collateral Circulation
Dextrans / administration & dosage*
Disease Models, Animal
Disease Progression
Esophageal and Gastric Varices / chemically induced,  physiopathology
Hypertension, Portal / chemically induced*,  physiopathology*,  radiography
Injections, Intravenous
Kidney Function Tests
Liver Circulation*
Liver Function Tests
Portal Pressure*
Portal Vein / physiopathology*,  radiography
Splenomegaly / chemically induced,  physiopathology
Time Factors
Vascular Resistance
Reg. No./Substance:
9004-54-0/Dextrans; 9014-76-0/sephadex

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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