Document Detail


A candidate reference method for uric acid in serum. I. Optimization and evaluation.
MedLine Citation:
PMID:  7055949     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We describe our optimization and evaluation of a candidate Reference Method for uric acid in serum. Reaction parameters were optimized for a manual, enzymic method for uric acid in which highly purified microbial uricase is used to quantitate uric acid by a differential ultraviolet procedure. We evaluated the method in terms of freedom from interferences, analytical recovery, precision, and comparison with five other uric acid methods. We conclude that (a) the candidate uric acid Reference Method exhibits the least interference; (b) all six methods exhibit satisfactory analytical recoveries and precision; and (c) results by all six methods agree well. As a result of this evaluation study, the manual ultraviolet uricase method for uric acid, with Tris as buffer, was chosen as the candidate Reference Method for uric acid.
Authors:
P H Duncan; N Gochman; T Cooper; E Smith; D Bayse
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinical chemistry     Volume:  28     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  1982 Feb 
Date Detail:
Created Date:  1982-04-22     Completed Date:  1982-04-22     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  284-90     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Autoanalysis
Evaluation Studies as Topic
Humans
Male
Middle Aged
Reference Standards
Spectrophotometry, Ultraviolet
Urate Oxidase / diagnostic use
Uric Acid / blood*
Chemical
Reg. No./Substance:
69-93-2/Uric Acid; EC 1.7.3.3/Urate Oxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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