|The cancer chemopreventive agent resveratrol induces tensin, a cell-matrix adhesion protein with signaling and antitumor activities.|
|PMID: 15735708 Owner: NLM Status: MEDLINE|
|During a search to identify resveratrol (3,5,4'-trihydroxy-trans-stilbene, RV) target genes in the human erythroleukemic K562 cell line, we show here that the tensin gene and protein levels are remarkably induced by this dietary polyphenol. Tensin, a cell-matrix adhesion protein binding the integrins and cytoskeletal actin filaments also interacts with PI3-kinase and JNK signaling pathways. Tensin induction by RV is associated with increased K562 cell adhesion to fibronectin, cell spreading and actin polymerization. The same responses were observed in the tensin-deficient MCF7 human breast cancer cell line. In K562 and MCF7 cells treated by RV, tensin was found in punctate and intracytoplasmic areas. In MCF7 epithelial cells, induction of tensin is not exclusively associated with plasma membrane-bound vinculin, suggesting a dual localization of tensin in both focal and fibrillar adhesions. Pharmacological blockade of PI3-kinase and Rho GTPases/Rho-kinase resulted in selective depletion of focal adhesions, disorganization of tensin localization and disruption of stress fibers. RV increased cell motility and attachment to fibronectin in MCF7 cells submitted to mechanical laminar flow stress, and abrogated estrogen-induced MCF7 cancer cell invasion. Our data support the conclusion that induction of tensin by RV contributes to the chemopreventive and anti-invasive activity of this natural dietary compound in tensin-negative and -deficient leukemic cells or epithelioid cancers.|
|Christelle M Rodrigue; Françoise Porteu; Nicole Navarro; Erik Bruyneel; Marc Bracke; Paul-Henri Romeo; Christian Gespach; Marie-Claude Garel|
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|Type: Journal Article; Research Support, Non-U.S. Gov't|
|Title: Oncogene Volume: 24 ISSN: 0950-9232 ISO Abbreviation: Oncogene Publication Date: 2005 May|
|Created Date: 2005-05-05 Completed Date: 2005-05-31 Revised Date: 2007-06-02|
Medline Journal Info:
|Nlm Unique ID: 8711562 Medline TA: Oncogene Country: England|
|Languages: eng Pagination: 3274-84 Citation Subset: IM|
|INSERM U567, UMR 8104, Département d'Hématologie, Institut Cochin, Université Paris V, Maternité Port-Royal, 123 boulevard de Port-Royal, Paris 75014, France.|
|APA/MLA Format Download EndNote Download BibTex|
Actins / metabolism
Anticarcinogenic Agents / pharmacology*
Antineoplastic Agents, Phytogenic / pharmacology*
Cell Adhesion Molecules / metabolism
Cell Line, Tumor
Cycloheximide / pharmacology
Cytoplasm / metabolism
Cytoskeleton / metabolism
DNA, Complementary / metabolism
Dose-Response Relationship, Drug
Enzyme Inhibitors / pharmacology*
Gene Expression Regulation, Neoplastic*
Microfilament Proteins / biosynthesis*
Neoplasms / prevention & control*
Protein Synthesis Inhibitors / pharmacology
RNA, Messenger / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Stilbenes / pharmacology*
|0/Actins; 0/Anticarcinogenic Agents; 0/Antineoplastic Agents, Phytogenic; 0/Cell Adhesion Molecules; 0/DNA, Complementary; 0/Enzyme Inhibitors; 0/Microfilament Proteins; 0/Protein Synthesis Inhibitors; 0/RNA, Messenger; 0/Stilbenes; 0/tensins; 501-36-0/resveratrol; 66-81-9/Cycloheximide; EC 220.127.116.11/1-Phosphatidylinositol 3-Kinase|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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