| The cancer chemopreventive agent resveratrol induces tensin, a cell-matrix adhesion protein with signaling and antitumor activities. | |
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MedLine Citation:
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PMID: 15735708 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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During a search to identify resveratrol (3,5,4'-trihydroxy-trans-stilbene, RV) target genes in the human erythroleukemic K562 cell line, we show here that the tensin gene and protein levels are remarkably induced by this dietary polyphenol. Tensin, a cell-matrix adhesion protein binding the integrins and cytoskeletal actin filaments also interacts with PI3-kinase and JNK signaling pathways. Tensin induction by RV is associated with increased K562 cell adhesion to fibronectin, cell spreading and actin polymerization. The same responses were observed in the tensin-deficient MCF7 human breast cancer cell line. In K562 and MCF7 cells treated by RV, tensin was found in punctate and intracytoplasmic areas. In MCF7 epithelial cells, induction of tensin is not exclusively associated with plasma membrane-bound vinculin, suggesting a dual localization of tensin in both focal and fibrillar adhesions. Pharmacological blockade of PI3-kinase and Rho GTPases/Rho-kinase resulted in selective depletion of focal adhesions, disorganization of tensin localization and disruption of stress fibers. RV increased cell motility and attachment to fibronectin in MCF7 cells submitted to mechanical laminar flow stress, and abrogated estrogen-induced MCF7 cancer cell invasion. Our data support the conclusion that induction of tensin by RV contributes to the chemopreventive and anti-invasive activity of this natural dietary compound in tensin-negative and -deficient leukemic cells or epithelioid cancers. |
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Authors:
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Christelle M Rodrigue; Françoise Porteu; Nicole Navarro; Erik Bruyneel; Marc Bracke; Paul-Henri Romeo; Christian Gespach; Marie-Claude Garel |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Oncogene Volume: 24 ISSN: 0950-9232 ISO Abbreviation: Oncogene Publication Date: 2005 May |
Date Detail:
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Created Date: 2005-05-05 Completed Date: 2005-05-31 Revised Date: 2007-06-02 |
Medline Journal Info:
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Nlm Unique ID: 8711562 Medline TA: Oncogene Country: England |
Other Details:
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Languages: eng Pagination: 3274-84 Citation Subset: IM |
Affiliation:
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INSERM U567, UMR 8104, Département d'Hématologie, Institut Cochin, Université Paris V, Maternité Port-Royal, 123 boulevard de Port-Royal, Paris 75014, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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1-Phosphatidylinositol 3-Kinase
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metabolism Actins / metabolism Anticarcinogenic Agents / pharmacology* Antineoplastic Agents, Phytogenic / pharmacology* Cell Adhesion Cell Adhesion Molecules / metabolism Cell Line, Tumor Cell Movement Cycloheximide / pharmacology Cytoplasm / metabolism Cytoskeleton / metabolism DNA, Complementary / metabolism Dose-Response Relationship, Drug Enzyme Inhibitors / pharmacology* Flow Cytometry Gene Expression Regulation, Neoplastic* Humans Immunoblotting K562 Cells Microfilament Proteins / biosynthesis* Neoplasm Invasiveness Neoplasms / prevention & control* Protein Synthesis Inhibitors / pharmacology RNA, Messenger / metabolism Reverse Transcriptase Polymerase Chain Reaction Signal Transduction Stilbenes / pharmacology* Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/Anticarcinogenic Agents; 0/Antineoplastic Agents, Phytogenic; 0/Cell Adhesion Molecules; 0/DNA, Complementary; 0/Enzyme Inhibitors; 0/Microfilament Proteins; 0/Protein Synthesis Inhibitors; 0/RNA, Messenger; 0/Stilbenes; 0/tensins; 501-36-0/resveratrol; 66-81-9/Cycloheximide; EC 2.7.1.137/1-Phosphatidylinositol 3-Kinase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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