Document Detail

The calcium-dependent cell-cell adhesion system regulates inner cell mass formation and cell surface polarization in early mouse development.
MedLine Citation:
PMID:  6652680     Owner:  NLM     Status:  MEDLINE    
The monoclonal antibody ECCD-1 inhibits Ca2+-dependent cell-cell adhesion in teratocarcinoma cells, recognizing a cell surface component of MW 124,000. When mouse embryos at various preimplantation stages were cultured in the presence of ECCD-1, the compacted morphology of the 8- to 16-cell-stage embryos was destroyed. In these embryos, cell proliferation normally occurred and development of blastocyst-like vesicles was attained. However, these embryos did not contain inner cell mass (ICM). We found that ECCD-1 affects the pattern of polarization of the cell surface in late 8- and 16-cell-stage blastomeres, as detected by staining with fluorescence-labeled concanavalin A. In normal blastomeres, the pole was always apart from the cell-cell contact plane. In those cultured in ECCD-1, formation of the pole tended to be inhibited, and if observed, the pole was close to the cell-cell contact plane. We discuss the possible mechanisms for inhibition of ICM formation caused by blocking Ca2+-dependent cell-cell adhesion between blastomeres.
Y Shirayoshi; T S Okada; M Takeichi
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell     Volume:  35     ISSN:  0092-8674     ISO Abbreviation:  Cell     Publication Date:  1983 Dec 
Date Detail:
Created Date:  1984-02-15     Completed Date:  1984-02-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0413066     Medline TA:  Cell     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  631-8     Citation Subset:  IM    
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MeSH Terms
Antibodies, Monoclonal
Blastomeres / cytology
Calcium / physiology
Cell Adhesion*
Cell Communication
Cell Membrane / physiology
Cleavage Stage, Ovum / cytology*
Membrane Proteins / immunology,  physiology
Molecular Weight
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Membrane Proteins; 7440-70-2/Calcium

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