| cGMP immunocytochemistry in aorta, kidney, retina and brain tissues of the rat after perfusion with nitroprusside. | |
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MedLine Citation:
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PMID: 2559068 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The distribution of cyclic guanosine 3',5' monophosphate (cGMP) producing cells in various organs of the rat were studied immunocytochemically using antibodies raised against formaldehyde-fixed cGMP. Sodium nitroprusside (SNP), a direct activator of guanylate cyclase and vasodilator, was used to enhance cGMP levels. In order to reach all organs optimally, whole body perfusion was performed using a modified Krebs-Ringer buffer at 37 degrees C, aerated with 5% CO2/95% O2, also containing isobutyl methyl xanthine (IBMX); a phosphodiesterase inhibitor. After 15-min pre-perfusion, SNP was added to the perfusate, followed by fast fixation with ice-cold 4% paraformaldehyde-phosphate buffer. After vehicle perfusion, only the retina showed cGMP immunoreactivity in the photoreceptor and ganglion layer, while other organs lacked cGMP immunoreactivity. After 15-min perfusion with SNP (10 microM), enhanced cGMP immunostaining was seen in smooth muscles of the aorta, amacrine-like cells in the retina, glomeruli of the kidney cortex, blood vessels in the dura mater, as well as cells in the pineal and in the median eminence. The results indicate that the distribution and the reactivity of cGMP producing cells, situated outside the blood brain barrier, can be studied by immunocytochemistry after pharmacological manipulations of the intact tissue with a nitrovasodilator using whole body perfusion. |
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Authors:
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H S Berkelmans; J Schipper; L Hudson; H W Steinbusch; J de Vente |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Histochemistry Volume: 93 ISSN: 0301-5564 ISO Abbreviation: Histochemistry Publication Date: 1989 |
Date Detail:
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Created Date: 1990-03-14 Completed Date: 1990-03-14 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0411300 Medline TA: Histochemistry Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 143-8 Citation Subset: IM |
Affiliation:
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Department of Pharmacology, Duphar B.V., Weesp, The Netherlands. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aorta / analysis*, drug effects Brain Chemistry* / drug effects Cyclic GMP / analysis* Kidney / analysis*, drug effects, ultrastructure Male Nitroprusside / pharmacology Perfusion / methods Rats Rats, Inbred Strains Retina / analysis*, drug effects, ultrastructure |
| Chemical | |
Reg. No./Substance:
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15078-28-1/Nitroprusside; 7665-99-8/Cyclic GMP |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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