Document Detail

cDNA microarray analysis of host-pathogen interactions in a porcine in vitro model for Toxoplasma gondii infection.
MedLine Citation:
PMID:  16790800     Owner:  NLM     Status:  MEDLINE    
Toxoplasma gondii induces the expression of proinflammatory cytokines, reorganizes organelles, scavenges nutrients, and inhibits apoptosis in infected host cells. We used a cDNA microarray of 420 annotated porcine expressed sequence tags to analyze the molecular basis of these changes at eight time points over a 72-hour period in porcine kidney epithelial (PK13) cells infected with T. gondii. A total of 401 genes with Cy3 and Cy5 spot intensities of >/=500 were selected for analysis, of which 263 (65.6%) were induced >/=2-fold (expression ratio, >/=2.0; P </= 0.05 [t test]) over at least one time point and 48 (12%) were significantly down-regulated. At least 12 functional categories of genes were modulated (up- or down-regulated) by T. gondii. The majority of induced genes were clustered as transcription, signal transduction, host immune response, nutrient metabolism, and apoptosis related. The expression of selected genes altered by T. gondii was validated by quantitative real-time reverse transcription-PCR. These results suggest that significant changes in gene expression occur in response to T. gondii infection in PK13 cells, facilitating further analysis of host-pathogen interactions in toxoplasmosis in a secondary host.
Margaret Okomo-Adhiambo; Craig Beattie; Anette Rink
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Infection and immunity     Volume:  74     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  2006 Jul 
Date Detail:
Created Date:  2006-06-22     Completed Date:  2006-08-24     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4254-65     Citation Subset:  IM    
Department of Animal Biotechnology, University of Nevada, 1664 North Virginia Street, Reno, NV 89557, USA.
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MeSH Terms
Cell Line
Disease Models, Animal
Gene Expression Profiling
Host-Parasite Interactions / immunology
Oligonucleotide Array Sequence Analysis*
Toxoplasma / immunology*
Toxoplasmosis, Animal / immunology,  parasitology

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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