Document Detail

cDNA cloning of a novel P-I lebetase isoform Le-4.
MedLine Citation:
PMID:  16098553     Owner:  NLM     Status:  MEDLINE    
In order to better understand the function of fibrinolytic enzyme lebetase isoforms and the synthesis of disintegrins we have isolated a cDNA encoding the most basic isoform (Le-4) from the cDNA library prepared from the poly(A)(+) RNA of the venomous gland of an individual Vipera lebetina snake. The truncated 5'-sequence of 1112 basepairs encodes the mature protein with 203 amino acid residues with calculated isoelectric point and size of 5.6 and 22,930 Da, respectively. Multiple comparison of the deduced amino acid sequence of the metalloprotease part of Le-4 is related to short reprolysins, identities were within the range of 60--87%. The two lebetase isoforms are synthesized in different way: Le-4 is synthesized with metalloprotease domain only; Le-3 is synthesized with metalloprotease and disintegrin-like domain and processed posttranslationally. The sequence of the disintegrin-like part of Le-3 is identical to A-chain of the heterodimeric disintegrin VLO5 from Vipera lebetina obtusa venom (Calvete et al., 2003).
Anu Aaspõllu; Jüri Siigur; Ene Siigur
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Toxicon : official journal of the International Society on Toxinology     Volume:  46     ISSN:  0041-0101     ISO Abbreviation:  Toxicon     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2005-09-12     Completed Date:  2006-02-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1307333     Medline TA:  Toxicon     Country:  England    
Other Details:
Languages:  eng     Pagination:  591-4     Citation Subset:  IM    
Department of Bioorganic Chemistry, National Institute of Chemical Physics and Biophysics, Akadeemia tee 23, 12618 Tallinn, Estonia.
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MeSH Terms
Amino Acid Sequence
Base Sequence
Cloning, Molecular
DNA, Complementary / biosynthesis*,  genetics
Disintegrins / chemistry
Isoelectric Focusing
Isoenzymes / biosynthesis,  genetics
Metalloendopeptidases / biosynthesis*,  genetics
Molecular Sequence Data
Protein Processing, Post-Translational
Viper Venoms / enzymology*
Reg. No./Substance:
0/DNA, Complementary; 0/Disintegrins; 0/Isoenzymes; 0/Viper Venoms; EC 3.4.24.-/Metalloendopeptidases; EC 3.4.24.-/lebetase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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