Document Detail

cDNA cloning of lymphotoxin alpha (LT-alpha) from a marsupial, Macropus eugenii.
MedLine Citation:
PMID:  10826697     Owner:  NLM     Status:  MEDLINE    
Lymphotoxin (LT) is a proinflammatory cytokine with a broad spectrum of immunological activities. While the 'classic' form of the molecule is a secreted homotrimer, now referred to in the literature as LT-alpha3, it has more recently been recognised that a membrane-bound form of LT exists on activated T lymphocytes and that this represents a complex between LT-alpha and a closely related type II membrane protein, LT-beta. Together with another related cytokine, tumour necrosis factor alpha (TNF-alpha), these molecules have been extremely well studied in eutherian mammals but not in any other group. Marsupials represent a distinct branch in mammalian evolution to that of eutherians, the two groups having diverged more than 100 million years ago. We report here for the first time, the cDNA cloning of LT-alpha from a marsupial, Macropus eugenii (tammar wallaby). This sequence was found to be relatively conserved when compared to orthologous sequences from eutherian mammals, sharing an average sequence identity of 70.4% at the nucleotide level and 71.7% at the deduced amino acid level.
G A Harrison; E M Deane
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  DNA sequence : the journal of DNA sequencing and mapping     Volume:  10     ISSN:  1042-5179     ISO Abbreviation:  DNA Seq.     Publication Date:  2000  
Date Detail:
Created Date:  2000-08-21     Completed Date:  2000-08-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9107800     Medline TA:  DNA Seq     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  399-403     Citation Subset:  IM    
Cooperative Research Centre for Conservation and Management of Marsupials. School of Science, University of Western Sydney, Nepean, NSW, Australia.
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MeSH Terms
Amino Acid Sequence
Base Sequence
Cloning, Molecular
DNA, Complementary / chemistry,  genetics*
Lymphotoxin-alpha / genetics*
Macropodidae / genetics*
Molecular Sequence Data
Sequence Alignment
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Reg. No./Substance:
0/DNA, Complementary; 0/Lymphotoxin-alpha

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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