Document Detail


cDNA cloning and functional characterization of Xenopus laevis DNase gamma.
MedLine Citation:
PMID:  16547842     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We report here the cDNA cloning and functional analysis of Xenopus DNase gamma (xDNase gamma). Two forms of cDNAs are isolated from adult spleen: one composing a 933 bp open reading frame for the enzymatically active xDNase gamma protein, and the other encoding an inactive short alternative form. Northern blot analysis revealed that the xDNase gamma mRNA is expressed in spleen, liver, testis, and ovary. xDNase gamma expression is scarcely detected in the tail muscle of tadpoles; however, it increases during metamorphosis and reaches a maximum during the late metamorphic climax. The ectopic expression of xDNase gamma results in the appearance of extensive DNA fragmentation in C2C12 myoblasts after the induction of apoptosis. In contrast, Xenopus DNase I fails to induce apoptotic DNA ladder formation under the same conditions. Our results suggest a possible involvement of xDNase gamma in apoptosis during amphibian metamorphosis.
Authors:
D Shiokawa; T Hatanaka; M Hatanaka; Y Shika; A Nishikawa; S Tanuma
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Apoptosis : an international journal on programmed cell death     Volume:  11     ISSN:  1360-8185     ISO Abbreviation:  Apoptosis     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2006-05-04     Completed Date:  2006-06-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9712129     Medline TA:  Apoptosis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  555-62     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, Japan.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AF059612
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MeSH Terms
Descriptor/Qualifier:
Alternative Splicing
Amino Acid Sequence
Animals
Apoptosis*
Base Sequence
Cell Line
Cloning, Molecular
DNA, Complementary / chemistry,  isolation & purification
Endodeoxyribonucleases / chemistry,  genetics*,  metabolism*
Larva / anatomy & histology,  growth & development
Metamorphosis, Biological
Mice
Molecular Sequence Data
RNA, Messenger / metabolism
Sequence Homology, Amino Acid
Xenopus Proteins / chemistry,  genetics*,  metabolism*
Xenopus laevis / genetics,  growth & development
Chemical
Reg. No./Substance:
0/DNA, Complementary; 0/RNA, Messenger; 0/Xenopus Proteins; EC 3.1.-/Endodeoxyribonucleases; EC 3.1.-/deoxyribonuclease gamma

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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