| cDNA cloning and functional characterization of Xenopus laevis DNase gamma. | |
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MedLine Citation:
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PMID: 16547842 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We report here the cDNA cloning and functional analysis of Xenopus DNase gamma (xDNase gamma). Two forms of cDNAs are isolated from adult spleen: one composing a 933 bp open reading frame for the enzymatically active xDNase gamma protein, and the other encoding an inactive short alternative form. Northern blot analysis revealed that the xDNase gamma mRNA is expressed in spleen, liver, testis, and ovary. xDNase gamma expression is scarcely detected in the tail muscle of tadpoles; however, it increases during metamorphosis and reaches a maximum during the late metamorphic climax. The ectopic expression of xDNase gamma results in the appearance of extensive DNA fragmentation in C2C12 myoblasts after the induction of apoptosis. In contrast, Xenopus DNase I fails to induce apoptotic DNA ladder formation under the same conditions. Our results suggest a possible involvement of xDNase gamma in apoptosis during amphibian metamorphosis. |
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Authors:
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D Shiokawa; T Hatanaka; M Hatanaka; Y Shika; A Nishikawa; S Tanuma |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Apoptosis : an international journal on programmed cell death Volume: 11 ISSN: 1360-8185 ISO Abbreviation: Apoptosis Publication Date: 2006 Apr |
Date Detail:
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Created Date: 2006-05-04 Completed Date: 2006-06-30 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9712129 Medline TA: Apoptosis Country: United States |
Other Details:
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Languages: eng Pagination: 555-62 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, Japan. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AF059612 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alternative Splicing Amino Acid Sequence Animals Apoptosis* Base Sequence Cell Line Cloning, Molecular DNA, Complementary / chemistry, isolation & purification Endodeoxyribonucleases / chemistry, genetics*, metabolism* Larva / anatomy & histology, growth & development Metamorphosis, Biological Mice Molecular Sequence Data RNA, Messenger / metabolism Sequence Homology, Amino Acid Xenopus Proteins / chemistry, genetics*, metabolism* Xenopus laevis / genetics, growth & development |
| Chemical | |
Reg. No./Substance:
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0/DNA, Complementary; 0/RNA, Messenger; 0/Xenopus Proteins; EC 3.1.-/Endodeoxyribonucleases; EC 3.1.-/deoxyribonuclease gamma |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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