Document Detail


cDNA cloning and expression of a human platelet-derived growth factor (PDGF) receptor specific for B-chain-containing PDGF molecules.
MedLine Citation:
PMID:  2850496     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The structure of the human receptor for platelet-derived growth factor (PDGF) has been deduced through cDNA cloning. A 5.45-kilobase-pair cDNA clone predicts a 1,106-amino-acid polypeptide, including the cleavable signal sequence. The overall amino acid sequence similarity with the murine PDGF receptor is 85%. After transcription of the cDNA and translation in vitro, a PDGF receptor antiserum was used to immunoprecipitate a product of predicted size, which also could be phosphorylated in vitro. Stable introduction of the cDNA into Chinese hamster ovary (CHO) cells led to the expression of a 190-kilodalton component, which was immunoprecipitated by the PDGF receptor antiserum; this most probably represents the mature PDGF receptor. Binding assays with different 125I-labeled dimeric forms of PDGF A and B chains showed that the PDGF receptor expressed in CHO cells bound PDGF-BB and, to a lesser extent, PDGF-AB, but not PDGF-AA.
Authors:
L Claesson-Welsh; A Eriksson; A Morén; L Severinsson; B Ek; A Ostman; C Betsholtz; C H Heldin
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Molecular and cellular biology     Volume:  8     ISSN:  0270-7306     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  1988 Aug 
Date Detail:
Created Date:  1989-02-23     Completed Date:  1989-02-23     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3476-86     Citation Subset:  IM    
Affiliation:
Ludwig Institute for Cancer Research, Uppsala Branch, Sweden.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/M21616
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Base Sequence
Cell Line
Cloning, Molecular*
DNA / genetics*
Genes
Humans
Macromolecular Substances
Mice
Molecular Sequence Data
Platelet-Derived Growth Factor / metabolism*
Protein Biosynthesis
Receptors, Cell Surface / genetics*,  metabolism
Receptors, Platelet-Derived Growth Factor
Sequence Homology, Nucleic Acid
Species Specificity
Transcription, Genetic*
Transfection
Chemical
Reg. No./Substance:
0/Macromolecular Substances; 0/Platelet-Derived Growth Factor; 0/Receptors, Cell Surface; 9007-49-2/DNA; EC 2.7.10.1/Receptors, Platelet-Derived Growth Factor
Comments/Corrections

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