| cDNA cloning of canine adrenomedullin and its gene expression in the heart and blood vessels in endotoxin shock. | |
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MedLine Citation:
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PMID: 9788655 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Adrenomedullin (AM) is a potent vasorelaxing peptide isolated from human pheochromocytoma. It is proposed that AM dilates the blood vessels by autocrine or paracrine mechanisms. Our investigation concerned whether the vasodilating function of AM accounts for the hypotension occurring in endotoxin shock. The effect of endotoxin shock on AM gene expression in the cardiovascular system was probed using a canine model. The cDNA encoding the AM precursor in dogs was isolated from cDNA libraries of the adrenal gland and was sequenced. The canine AM precursor is constituted of 188 amino acids and its AM consists of 52 amino acids, similar to human and porcine AM. The dogs were injected intravenously with 2 mg/kg of lipopolysaccharide (LPS) after being anesthetized and intubated. After 4 h, we collected whole blood, the large and small blood vessels, and the heart from our canine model of endotoxin shock, and extracted RNA from those tissues for Northern blot analysis. In the endotoxin shock model, the mRNA levels of the AM had increased in almost all of the blood vessels. These results suggest that AM may dilate the blood vessels systemically, even if AM acts as a local modulator of vascular tone. In addition, the plasma concentrations of the AM were high enough to allow for AM to act as a vasodilating hormone. Consequently, AM may be one of the factors facilitating severe hypotension complicated with endotoxin shock. |
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Authors:
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Y Ono; M Kojima; K Okada; K Kangawa |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Shock (Augusta, Ga.) Volume: 10 ISSN: 1073-2322 ISO Abbreviation: Shock Publication Date: 1998 Oct |
Date Detail:
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Created Date: 1998-12-23 Completed Date: 1998-12-23 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9421564 Medline TA: Shock Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 243-7 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, National Cardiovascular Center Research Institute, Suita, Osaka, Japan. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/U96127 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adrenomedullin Amino Acid Sequence Animals Base Sequence Blood Vessels / metabolism* Cloning, Molecular DNA, Complementary Dogs Female Gene Expression Regulation Humans Molecular Sequence Data Myocardium / metabolism* Peptides / genetics*, metabolism* Sequence Analysis, DNA Sequence Homology, Amino Acid Shock, Septic / genetics*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/DNA, Complementary; 0/Peptides; 148498-78-6/Adrenomedullin |
| Comments/Corrections | |
Comment In:
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Shock. 1998 Nov;10(5):383-4
[PMID:
9840656
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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