Document Detail

cDNA clone encoding a high molecular weight antigen of Babesia bovis.
MedLine Citation:
PMID:  3033495     Owner:  NLM     Status:  MEDLINE    
An expression library was constructed by inserting cDNA copied from mRNA of the blood stages of Babesia bovis isolate KA into bacteriophage lambda gt11-amp3. An antigen-positive cDNA clone detected by screening the library with antibodies from cattle vaccinated with the KA isolate was shown to encode part of a high-molecular weight polypeptide antigen of B. bovis. This molecule was a dominant immunogen and was found by immunofluorescence to be within the parasite in infected erythrocytes.
A Gill; P Timms; D J Kemp
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular and biochemical parasitology     Volume:  22     ISSN:  0166-6851     ISO Abbreviation:  Mol. Biochem. Parasitol.     Publication Date:  1987 Jan 
Date Detail:
Created Date:  1987-05-26     Completed Date:  1987-05-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8006324     Medline TA:  Mol Biochem Parasitol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  195-202     Citation Subset:  IM    
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MeSH Terms
Antigens, Protozoan / analysis,  genetics*
Babesia / genetics*,  immunology
Cloning, Molecular
DNA / genetics*
DNA Restriction Enzymes
DNA Transposable Elements
Deoxyribonuclease EcoRI
Electrophoresis, Polyacrylamide Gel
Erythrocytes / parasitology
Nucleic Acid Hybridization
Peptides / analysis,  genetics*
RNA, Messenger / genetics
Reg. No./Substance:
0/Antigens, Protozoan; 0/DNA Transposable Elements; 0/Peptides; 0/RNA, Messenger; 9007-49-2/DNA; EC 3.1.21.-/DNA Restriction Enzymes; EC 3.1.21.-/Deoxyribonuclease EcoRI

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