Document Detail


cDNA AFLP-based techniques for studying transcript profiles in horses.
MedLine Citation:
PMID:  15924927     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The identification of differentially expressed genes is a fundamental prerequisite for understanding the molecular regulation of most physiological and pathological processes. Among the procedures employed to compare mRNA populations, those that are gel-based appear to hold great promise and are considered excellent tools for studying gene expression in species, such as the equine one, for which little genomic information is available. In the present study, we evaluated two techniques for studying mRNA profiles in horse tissue, one referred to the cDNA-amplified fragment length polymorphism (AFLP) that we called C-AFLP (classical cDNA-AFLP) protocol and the other to ordered differential display (ODD) with some modifications that we named S-AFLP (systematic cDNA-AFLP). Both techniques can be applied in live animals because of the small amount of sample required. We applied the S-AFLP to investigate horse transcript profile modifications during physical exercise. We found two transcripts that are mostly expressed during exercise and immediately after the end of it.
Authors:
K Cappelli; A Porceddu; A Verini-Supplizi; S Capomaccio; F De Marchis; M Falcinelli; A Gaiti; M Silvestrelli
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-01-18
Journal Detail:
Title:  Research in veterinary science     Volume:  79     ISSN:  0034-5288     ISO Abbreviation:  Res. Vet. Sci.     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2005-05-31     Completed Date:  2005-08-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0401300     Medline TA:  Res Vet Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  105-12     Citation Subset:  IM    
Affiliation:
Centro di Studio del Cavallo Sportivo, Facoltà di Medicina Veterinaria, University of Perugia, Via San Costanzo 4, 06126 Perugia, Italy. vete7@unipg.it
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MeSH Terms
Descriptor/Qualifier:
Animals
DNA, Complementary*
Gene Expression Profiling / veterinary*
Horses / genetics*
Polymorphism, Genetic
RNA, Messenger / analysis
Chemical
Reg. No./Substance:
0/DNA, Complementary; 0/RNA, Messenger

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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