| cAMP regulation of vasopressin mRNA content in hypothalamo-neurohypophysial explants. | |
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MedLine Citation:
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PMID: 8853375 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Stimulation of vasopressin (VP) gene expression by adenosine 3',5'-cyclic monophosphate (cAMP) has been observed in dispersed hypothalamic cultures, in VP-expressing cell lines, and in cells transfected with reporter genes regulated by the VP gene promoter. However, treatment of hypothalamo-neurohypophysial system (HNS) explants with forskolin (25 microM), an activator of adenyl cyclase, and 3-isobutyl-1-methylxanthine (IBMX; 500 microM), a phosphodiesterase inhibitor, resulted in a decrease in VP mRNA. Time course analysis revealed that IBMX and forskolin reduced the VP mRNA content to 50% of control explants after 8 and 12 h despite a dramatic stimulation of VP release. This effect was due to the activation of adenyl cyclase by forskolin, because neither IBMX alone nor the inactive analogue of forskolin, 1,9-dideoxyforskolin, decreased VP mRNA content. In contrast, 8-bromoadenosine 3',5'-cyclic monophosphate and the D1 dopamine receptor agonist, SKF-38393, increased VP mRNA content, but these agents were less potent in stimulating VP release, suggesting a concentration dependency of the forskolin effect. This was confirmed when forskolin (10 microM) was found to increase VP mRNA content. Thus receptor-mediated activation of adenyl cyclase results in an increase in VP mRNA content. |
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Authors:
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C D Sladek; K Y Fisher; H E Sidorowicz; J R Mathiasen |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The American journal of physiology Volume: 271 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1996 Sep |
Date Detail:
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Created Date: 1996-12-05 Completed Date: 1996-12-05 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: R554-60 Citation Subset: IM |
Affiliation:
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Department of Physiology, Finch University of Health Sciences, Chicago Medical School, Illinois 60064, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine
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pharmacology Animals Culture Techniques Cyclic AMP / physiology* Dopamine Agonists / pharmacology Forskolin / pharmacology Hypothalamo-Hypophyseal System / drug effects, metabolism* Male Osmolar Concentration Pituitary Gland, Posterior / drug effects, metabolism* RNA, Messenger / metabolism* Rats Rats, Sprague-Dawley Stimulation, Chemical Time Factors Vasopressins / genetics* |
| Grant Support | |
ID/Acronym/Agency:
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NS-27975/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Dopamine Agonists; 0/RNA, Messenger; 11000-17-2/Vasopressins; 60-92-4/Cyclic AMP; 66428-89-5/Forskolin; 67287-49-4/2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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