| cAMP regulates vegetative growth and cell cycle in Candida albicans. | |
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MedLine Citation:
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PMID: 17557192 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We demonstrate here the regulatory role of cAMP in cell cycle of Candida albicans. cAMP was found to be a positive signal for growth and morphogenesis. Phosphodiesterase inhibitor aminophylline exhibited significant effects, i.e., increased growth, as well as induced morphogenesis. Atropine and trifluoperazine negatively regulated (inhibited) growth and did not induce morphogenesis. These changes were attributed to increase in cAMP levels and protein kinase A (PKA) activity in presence of aminophylline, while reduction was observed in atropine and trifluoperazine (TFP) grown cells. Alteration in cAMP signaling pathway affected the cell cycle progression in Candida albicans. Increased cAMP levels in aminophylline grown cells reduced the duration of cell cycle by inciting the cell cycle-specific expression of G1 cyclins (CLN1 and CLN2). However atropine and trifluoperazine delayed the expression of G1 cyclins and hence prolonged the cell cycle. Implication of cAMP signaling pathway in both the cell cycle and morphogenesis further opened the channels to explore the potential of this pathway to serve as a target for development of new antifungal drugs. |
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Authors:
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Ajay Singh; Sadhna Sharma; Gopal K Khuller |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-06-08 |
Journal Detail:
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Title: Molecular and cellular biochemistry Volume: 304 ISSN: 0300-8177 ISO Abbreviation: Mol. Cell. Biochem. Publication Date: 2007 Oct |
Date Detail:
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Created Date: 2007-09-20 Completed Date: 2008-02-05 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0364456 Medline TA: Mol Cell Biochem Country: Netherlands |
Other Details:
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Languages: eng Pagination: 331-41 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Post Graduate Institute of Medical Education & Research (PGIMER), Chandigarh 160012, India. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aminophylline
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pharmacology Atropine / pharmacology Candida albicans / cytology*, drug effects, metabolism, physiology Cell Cycle* / drug effects Cell Proliferation* / drug effects Cyclic AMP / metabolism, physiology* Cyclic AMP-Dependent Protein Kinases / metabolism, physiology Signal Transduction / physiology Trifluoperazine / pharmacology |
| Chemical | |
Reg. No./Substance:
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117-89-5/Trifluoperazine; 317-34-0/Aminophylline; 51-55-8/Atropine; 60-92-4/Cyclic AMP; EC 2.7.11.11/Cyclic AMP-Dependent Protein Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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