| cAMP and protein kinase A stimulate acidification of rat liver endosomes in the absence of chloride. | |
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MedLine Citation:
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PMID: 8670202 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Endosomes and lysosomes are acidified by an electrogenic proton pump in parallel with a chloride conductance and in kidney both of these may be regulated by cAMP. In vitro exposure of isolated rat liver endosomes to cAMP, PKA and GTP-gamma S stimulated acidification of "early" endosomes with or without C1-, but not in the absence of K+. cAMP and PKA also increased acidification rates of purified "late" endosomes, multivesicular bodies, CURL vesicles and lysosomes. "Early" endosomes prepared from livers perfused with cAMP also exhibited increased rates of acidification. cAMP and PKA had no consistent effects on steady-state intravesicular pH or proton efflux rates. Thus, acidification of several types of liver endocytic vesicles was stimulated by cAMP and PKA in the presence and absence of chloride, possibly due to changes in the proton pump itself and/or a cation conductance. |
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Authors:
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R W Van Dyke; K V Root; R A Hsi |
Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 222 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1996 May |
Date Detail:
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Created Date: 1996-08-06 Completed Date: 1996-08-06 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 312-6 Citation Subset: IM |
Affiliation:
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Department of Medicine, University of Michigan School of Medicine, Ann Arbor 48109-0682, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bucladesine / pharmacology Chlorides / pharmacology* Cyclic AMP / metabolism* Cyclic AMP-Dependent Protein Kinases / metabolism*, pharmacology* Dextrans Endosomes / drug effects, metabolism* Ethinyl Estradiol / pharmacology Fluorescein-5-isothiocyanate / analogs & derivatives Fluorescent Dyes Guanosine 5'-O-(3-Thiotriphosphate) / pharmacology Guanosine Diphosphate / metabolism Guanosine Triphosphate / metabolism Hydrogen-Ion Concentration Kinetics Liver / metabolism* Lysosomes / drug effects, metabolism Macromolecular Substances Rats |
| Grant Support | |
ID/Acronym/Agency:
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R01 DK-38333/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Chlorides; 0/Fluorescent Dyes; 0/Macromolecular Substances; 0/fluorescein isothiocyanate dextran; 146-91-8/Guanosine Diphosphate; 3326-32-7/Fluorescein-5-isothiocyanate; 362-74-3/Bucladesine; 37589-80-3/Guanosine 5'-O-(3-Thiotriphosphate); 57-63-6/Ethinyl Estradiol; 60-92-4/Cyclic AMP; 86-01-1/Guanosine Triphosphate; 9004-54-0/Dextrans; EC 2.7.11.11/Cyclic AMP-Dependent Protein Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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