Document Detail


cAMP-mediated growth inhibition of a B-lymphoid precursor cell line Reh is associated with an early transient delay in G2/M, followed by an accumulation of cells in G1.
MedLine Citation:
PMID:  2848044     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study was undertaken to gain more insight into the effects of cyclic adenosine monophosphate (cAMP) on cell-cycle progression in the B-lymphoid precursor cell line Reh. The adenylate cyclase activator forskolin reduced the proliferation of asynchronously growing Reh cells by 50% after 72 hr culture. Growth inhibition was associated with an accumulation of cells in G1. Furthermore, we demonstrated that forskolin provoked a delay of cells for approximately 10 hr in G2/M prior to the G1 arrest. Two different methods were applied to elucidate how cells in different phases of the cell cycle were affected by an elevated cAMP level. One method was based on centrifugal elutriation, whereby synchronous cell populations from the different phases of the cell cycle were isolated. By the other method, S-phase cells were selectively stained by pulsing asynchronously growing cells with bromo-deoxyuridine (BrdU). The data demonstrate that the position of a cell in the cell cycle is critical in determining how the cell will respond to an elevated cAMP level. Thus cells in G1 at the time forskolin is added are not delayed in G2/M, but they will subsequently accumulate in G1 after 48 hr. Cells given forskolin in G2/m, however, are delayed for 10 hr in G2/M, but they do not accumulate in G1. Cells given forskolin in the S phase are delayed in G2/M as well as arrested in G1. The results suggest that cAMP inhibits growth of the Reh cells by preventing the cells from passing important restriction points located in the G1 and G2 phases of the cell cycle.
Authors:
H K Blomhoff; R Blomhoff; T Stokke; C deLange Davies; K Brevik; E B Smeland; S Funderud; T Godal
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of cellular physiology     Volume:  137     ISSN:  0021-9541     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  1988 Dec 
Date Detail:
Created Date:  1989-01-12     Completed Date:  1989-01-12     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  583-7     Citation Subset:  IM    
Affiliation:
Department of Pathology, The Norwegian Radium Hospital, Oslo.
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MeSH Terms
Descriptor/Qualifier:
B-Lymphocytes / cytology*,  drug effects
Cell Line
Cyclic AMP / physiology*
DNA / analysis
Forskolin / pharmacology*
Interphase* / drug effects
Mitosis* / drug effects
Chemical
Reg. No./Substance:
60-92-4/Cyclic AMP; 66428-89-5/Forskolin; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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