Document Detail

cAMP-independent responses of olfactory neurons in Xenopus laevis tadpoles and their projection onto olfactory bulb neurons.
MedLine Citation:
PMID:  12456827     Owner:  NLM     Status:  MEDLINE    
We report on responses of olfactory receptor neurons (ORNs) upon application of amino acids and forskolin using a novel slice preparation of the olfactory epithelium of Xenopus laevis tadpoles. Responses were measured using the patch-clamp technique. Both amino acids and forskolin proved to be potent stimuli. Interestingly, a number of ORNs that responded to amino acids did not respond to forskolin. This suggests that some amino acids activate transduction pathways other than the well-known cAMP-mediated one. The differential processing of cAMP-mediated stimuli on the one hand and amino acid stimuli on the other was further elucidated by calcium-imaging of olfactory bulb neurons using a novel nose-olfactory bulb preparation of Xenopus laevis tadpoles. The projection pattern of amino acid-sensitive ORNs to olfactory bulb neurons differed markedly from the projection pattern of forskolin-sensitive ORNs. Olfactory bulb neurons activated by amino acids were located laterally compared to those activated by forskolin, and only a small proportion responded to both stimuli. The ensemble of neurons activated by forskolin was also activated by the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX) and the membrane-permeant cAMP analogue 8-(4-chlorophenylthio)adenosine 3',5'-cyclic monophosphate (pCPT-cAMP). We therefore conclude that sensory transduction of a number of amino acids is cAMP independent, and amino acid- and cAMP-mediated responses are processed differentially at the level of the olfactory bulb.
Ivan Manzini; Wolfgang Rössler; Detlev Schild
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  The Journal of physiology     Volume:  545     ISSN:  0022-3751     ISO Abbreviation:  J. Physiol. (Lond.)     Publication Date:  2002 Dec 
Date Detail:
Created Date:  2002-11-28     Completed Date:  2003-06-16     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  0266262     Medline TA:  J Physiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  475-84     Citation Subset:  IM    
Physiologisches Institut, Universität Göttingen, Humboldtallee 23, 37073 Göttingen, Germany.
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MeSH Terms
Amino Acids / pharmacology
Cyclic AMP / physiology*
Forskolin / pharmacology
Membrane Potentials / drug effects,  physiology
Neurons / drug effects,  physiology*
Nose / innervation,  physiology
Olfactory Bulb / cytology,  drug effects,  physiology*
Olfactory Pathways / cytology,  drug effects,  physiology*
Olfactory Receptor Neurons / drug effects,  physiology*
Patch-Clamp Techniques
Signal Transduction / drug effects,  physiology
Xenopus laevis
Reg. No./Substance:
0/Amino Acids; 60-92-4/Cyclic AMP; 66428-89-5/Forskolin

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