| cAMP-independent responses of olfactory neurons in Xenopus laevis tadpoles and their projection onto olfactory bulb neurons. | |
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MedLine Citation:
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PMID: 12456827 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We report on responses of olfactory receptor neurons (ORNs) upon application of amino acids and forskolin using a novel slice preparation of the olfactory epithelium of Xenopus laevis tadpoles. Responses were measured using the patch-clamp technique. Both amino acids and forskolin proved to be potent stimuli. Interestingly, a number of ORNs that responded to amino acids did not respond to forskolin. This suggests that some amino acids activate transduction pathways other than the well-known cAMP-mediated one. The differential processing of cAMP-mediated stimuli on the one hand and amino acid stimuli on the other was further elucidated by calcium-imaging of olfactory bulb neurons using a novel nose-olfactory bulb preparation of Xenopus laevis tadpoles. The projection pattern of amino acid-sensitive ORNs to olfactory bulb neurons differed markedly from the projection pattern of forskolin-sensitive ORNs. Olfactory bulb neurons activated by amino acids were located laterally compared to those activated by forskolin, and only a small proportion responded to both stimuli. The ensemble of neurons activated by forskolin was also activated by the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX) and the membrane-permeant cAMP analogue 8-(4-chlorophenylthio)adenosine 3',5'-cyclic monophosphate (pCPT-cAMP). We therefore conclude that sensory transduction of a number of amino acids is cAMP independent, and amino acid- and cAMP-mediated responses are processed differentially at the level of the olfactory bulb. |
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Authors:
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Ivan Manzini; Wolfgang Rössler; Detlev Schild |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: The Journal of physiology Volume: 545 ISSN: 0022-3751 ISO Abbreviation: J. Physiol. (Lond.) Publication Date: 2002 Dec |
Date Detail:
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Created Date: 2002-11-28 Completed Date: 2003-06-16 Revised Date: 2013-06-09 |
Medline Journal Info:
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Nlm Unique ID: 0266262 Medline TA: J Physiol Country: England |
Other Details:
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Languages: eng Pagination: 475-84 Citation Subset: IM |
Affiliation:
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Physiologisches Institut, Universität Göttingen, Humboldtallee 23, 37073 Göttingen, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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pharmacology Animals Cyclic AMP / physiology* Forskolin / pharmacology Histocytochemistry Larva Membrane Potentials / drug effects, physiology Neurons / drug effects, physiology* Nose / innervation, physiology Odors Olfactory Bulb / cytology, drug effects, physiology* Olfactory Pathways / cytology, drug effects, physiology* Olfactory Receptor Neurons / drug effects, physiology* Patch-Clamp Techniques Signal Transduction / drug effects, physiology Xenopus laevis |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 60-92-4/Cyclic AMP; 66428-89-5/Forskolin |
| Comments/Corrections | |
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