Document Detail


The cAMP-dependent signalling cascade in the two luteal cell types of the pregnant rat corpus luteum.
MedLine Citation:
PMID:  1321769     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Corpora lutea of rats, like those of many other species, contain two sub-populations of luteal cells. In this report we sought to determine whether the luteinizing hormone (LH)- and beta-adrenergic cAMP signal transduction pathways known to be present in rat corpora lutea were segregated into separate luteal cell types. Results showed that large rat luteal cells, obtained on day 3 of pregnancy, exhibited elevated LH- and most notably epinephrine-stimulated adenylyl cyclase activities but equivalent cAMP-dependent catalytic protein kinase and total regulatory subunit cAMP binding activities compared to small luteal cells. Progesterone production by the large cell was greater than that by the small cell but both cells were equally sensitive to stimulation of progesterone by LH. However, neither the large nor the small rat luteal cell produced significant progesterone in response to epinephrine despite a marked epinephrine-stimulated adenylyl cyclase in both cell populations. The LH-stimulated progesterone synthetic response of the two sub-populations of rat luteal cells is more similar to that of the developing monkey corpus luteum and contrasts sharply with that of ruminants.
Authors:
S E Nelson; G Gibori; M Hunzicker-Dunn
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular and cellular endocrinology     Volume:  85     ISSN:  0303-7207     ISO Abbreviation:  Mol. Cell. Endocrinol.     Publication Date:  1992 Jun 
Date Detail:
Created Date:  1992-08-26     Completed Date:  1992-08-26     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  7500844     Medline TA:  Mol Cell Endocrinol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  195-203     Citation Subset:  IM    
Affiliation:
Department of Physiology and Biophysics, University of Illinois College of Medicine, IL 60612.
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MeSH Terms
Descriptor/Qualifier:
Adenylate Cyclase / metabolism
Animals
Cells, Cultured
Corpus Luteum / cytology,  drug effects,  metabolism*
Cyclic AMP / metabolism,  physiology*
Epinephrine / pharmacology
Female
Forskolin / pharmacology
Luteinizing Hormone / physiology
Pregnancy
Pregnancy, Animal / metabolism*
Progesterone / biosynthesis
Protein Kinases / metabolism
Rats
Signal Transduction / physiology*
Grant Support
ID/Acronym/Agency:
HD 11119/HD/NICHD NIH HHS; HD 21921/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
51-43-4/Epinephrine; 57-83-0/Progesterone; 60-92-4/Cyclic AMP; 66428-89-5/Forskolin; 9002-67-9/Luteinizing Hormone; EC 2.7.-/Protein Kinases; EC 4.6.1.1/Adenylate Cyclase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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