| c-myb has a character of oxidative stress resistance in aged human diploid fibroblasts: regulates SAPK/JNK and Hsp60 pathway consequently. | |
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MedLine Citation:
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PMID: 19707884 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study examined whether c-myb acts as a survival molecule in aged cells. A previous in vitro ageing model suggested that aged cells have a higher cell capacity for survival after exposure to oxidative stress, which involves blockage of the translocation of Hsp60 from the mitochondria to the cytoplasm followed by SAPK/JNK inactivation, than young cells. In human diploid fibroblasts (HDFs), c-myb expression increased gradually with ageing, and this increase had a significant influence on the cell survival capacity after exposure to oxidative stress. To clarify the role of c-myb in oxidative stress, young cells under 21 passages, which lacked c-myb expression, were transfected with adenovirus-mediated c-myb for express c-myb. These c-myb-over-expressed young cells showed increased cell viability upon exposure to oxidative stress to a similar extent to that of the aged cells. In addition, these c-myb-over-expressed young cells did not exhibit SAPK/JNK activation, Hsp60 displacement and cytochrome C release, as was observed in aged cells. The aged cells that had c-myb suppressed using siRNA c-myb showed reduced cell viability and increased apoptosis in a manner to that observed in young cells. From this study, c-myb blocked SAPK/JNK and Hsp60 translocation upon exposure to oxidative stress. This result suggests that c-myb might act as a modulator of cell survival in the ageing process by suppressing apoptosis in aged cells. |
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Authors:
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Young-Hee Lee; Nan-Hee Lee; Govinda Bhattarai; Pyong-Han Hwang; Tae-Il Kim; Eun-Chung Jhee; Ho-Keun Yi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-08-27 |
Journal Detail:
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Title: Biogerontology Volume: 11 ISSN: 1389-5729 ISO Abbreviation: Biogerontology Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-04-27 Completed Date: 2010-07-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100930043 Medline TA: Biogerontology Country: Netherlands |
Other Details:
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Languages: eng Pagination: 267-74 Citation Subset: IM |
Affiliation:
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Department of Oral Biochemistry and Institute of Oral-Bio Science, BK21 program, School of Dentistry, Chonbuk National University, Jeonju, Jeonbuk, 561-712, Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aging
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metabolism* Apoptosis Base Sequence Blotting, Western Cells, Cultured Chaperonin 60 / metabolism* Diploidy* Fibroblasts / metabolism Humans MAP Kinase Kinase 4 / metabolism* Mitogen-Activated Protein Kinases / metabolism* Oxidative Stress* Proto-Oncogene Proteins c-myb / metabolism* RNA, Small Interfering |
| Chemical | |
Reg. No./Substance:
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0/Chaperonin 60; 0/Proto-Oncogene Proteins c-myb; 0/RNA, Small Interfering; EC 2.7.11.24/Mitogen-Activated Protein Kinases; EC 2.7.12.2/MAP Kinase Kinase 4 |
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