Document Detail

c-myb effects on kinetic events during MEL cell differentiation.
MedLine Citation:
PMID:  1533276     Owner:  NLM     Status:  MEDLINE    
During dimethylsulfoxide (DMSO)-induced differentiation of Friend mouse erythroleukemia (MEL) cells there is a biphasic fall in c-myb mRNA levels. We have previously shown that constitutive expression of c-myb blocks differentiation. To delineate more accurately the point at which Myb blocks differentiation, MEL cells were transfected with a human c-myb construct under the control of the beta-globin promoter and enhancers. In concert with endogenous DMSO-induced globin transcription during MEL cell differentiation, the beta-globin c-myb transcription unit of the transfected plasmid is activated after 3-5 days of culture in media containing DMSO. Here we describe c-myb-transformed MEL clones which undergo delayed expression of the exogenous c-myb following 3-5 days of culture in DMSO. In contrast to wild-type MEL cells, both clones failed to display phenotypic markers of differentiation and continued to proliferate for up to 10 days of culture. These data suggest that the late fall in c-myb levels may be required in order for differentiation to occur. Additionally, we suggest that constitutive expression of c-myb does not block early commitment events such as activation of histone Hl', subsequent chromatin condensation, and alteration of proliferation-related gene expression. Taken together, these results show that c-myb acts very late in the process of differentiation.
R Danish; O el-Awar; B L Weber; J Langmore; L A Turka; J J Ryan; M F Clarke
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Oncogene     Volume:  7     ISSN:  0950-9232     ISO Abbreviation:  Oncogene     Publication Date:  1992 May 
Date Detail:
Created Date:  1992-05-28     Completed Date:  1992-05-28     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8711562     Medline TA:  Oncogene     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  901-7     Citation Subset:  IM    
Department of Internal Medicine, University of Michigan Medical Center, Ann Arbor 48109-0668.
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MeSH Terms
Blotting, Northern
Cell Differentiation / genetics*
Cell Line
Cyclins / analysis
DNA Polymerase II / biosynthesis
Dimethyl Sulfoxide / pharmacology
Gene Expression / drug effects
Heme / biosynthesis
Histones / biosynthesis
Leukemia, Erythroblastic, Acute / metabolism,  pathology*
Oncogenes / physiology*
Proto-Oncogene Proteins / biosynthesis
Proto-Oncogene Proteins c-myb
Restriction Mapping
Grant Support
Reg. No./Substance:
0/Cyclins; 0/Histones; 0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-myb; 14875-96-8/Heme; 67-68-5/Dimethyl Sulfoxide; EC 2.7.7.-/DNA Polymerase II

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