Document Detail

c-fos expression in the forebrain and brainstem of White Leghorn hens following osmotic and cardiovascular challenges.
MedLine Citation:
PMID:  10470493     Owner:  NLM     Status:  MEDLINE    
In chickens, hyperosmolality and hemorrhage increase hypothalamic vasotocin (AVT) gene expression and stimulate the secretion of AVT from the posterior pituitary gland. In this study, c-fos expression was used to identify areas in the forebrain and brainstem of the domestic chicken that are activated following acute osmotic stress and hemorrhage-induced hypotension. Conscious hens were osmotically stimulated by administering a single intraperitoneal injection of 3 M NaCl (5 ml/kg). Urethane-anesthetized hens were bled to a mean systemic arterial pressure of 80-90 mm Hg and maintained at this blood pressure for 1 h with additional bleedings as required. In both studies, the expression of c-fos was determined in control and experimental birds by using Northern blot analysis and in situ hybridization analysis. Osmotic stress and hemorrhage-induced hypotension increased c-fos expression in the same brain regions. Prominent structures in the forebrain that expressed c-fos mRNA following acute osmotic stress and hemorrhage-induced hypotension included the supraoptic nucleus and paraventricular nucleus and nuclei within the hypothalamus that are anterior and ventral to the third ventricle. In the chicken, this region includes the organum subseptale, the o. vasculosum laminae terminalis, and the nucleus septalis medialis. In the brainstem, following either injection of 3 M NaCl or hemorrhage-induced hypotension, increased c-fos expression was observed in the nucleus of the solitary tract, parabrachial nucleus, area postrema, and locus ceruleus. Thus, the chicken central nervous system appears to use shared neuronal circuitry to stimulate hypothalamic AVT release in response to disturbances in body fluid composition and decreases in either systemic blood pressure or volume.
S Jaccoby; T I Koike; L E Cornett
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Cell and tissue research     Volume:  297     ISSN:  0302-766X     ISO Abbreviation:  Cell Tissue Res.     Publication Date:  1999 Aug 
Date Detail:
Created Date:  1999-10-13     Completed Date:  1999-10-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0417625     Medline TA:  Cell Tissue Res     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  229-39     Citation Subset:  IM    
Department of Physiology and Biophysics, University of Arkansas for Medical Sciences, Little Rock 72205, USA.
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MeSH Terms
Brain Stem / metabolism*
Gene Expression Regulation / physiology*
Hemorrhage / complications,  metabolism
Hypotension / etiology,  metabolism
In Situ Hybridization
Nerve Tissue Proteins / genetics*
Osmotic Pressure
Prosencephalon / metabolism*
Proto-Oncogene Proteins c-fos / genetics*
Reg. No./Substance:
0/Nerve Tissue Proteins; 0/Proto-Oncogene Proteins c-fos

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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