Document Detail


c-erbA and v-erbA modulate growth and gene expression of a mouse glial precursor cell line.
MedLine Citation:
PMID:  7947384     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The c-erbA alpha protooncogene coding for the thyroid hormone (T3) receptor (TR alpha 1) and the viral, mutated v-erbA oncogene were expressed in an immortal mouse glial cell line (B3.1) using retroviral vectors. c-erbA alpha expression led to a decrease in cell proliferation in high and low serum conditions, both in the presence and in the absence of T3. In serum-free medium, c-erbA-expressing cells (B3.1 + TR alpha 1) were completely arrested, whereas cells expressing v-erbA (B3.1 + v-erbA) showed a higher DNA synthesis rate than normal B3.1 cells. Although proliferation of all three cell types was stimulated by platelet-derived growth factor and basic fibroblast growth factor, differences were also observed in the response to these agents. B3.1 + TR alpha 1 cells were more sensitive to platelet-derived growth factor than B3.1 and B3.1 + v-erbA cells. In contrast, B3.1 cells responded to basic fibroblast growth factor better than B3.1 + TR alpha 1 or B3.1 + v-erbA cells. Insulin-like growth factor I potentiated the action of platelet-derived growth factor and basic fibroblast growth factor. Again, different responses to treatment with insulin-like growth factor I alone were observed; B3.1 + TR alpha 1 cells did not respond to it, whereas B3.1 + v-erbA cells showed a dramatic stimulation by this agent. Interestingly, in the presence of T3, the blockade in B3.1 + TR alpha 1 cell proliferation was accompanied by the down-regulation of the typical astrocytic genes, glial fibrillary acidic protein and vimentin. These hormone effects were not found in v-erbA-expressing cells. In addition, v-erbA inhibited the basal expression of the cyclic nucleotide phosphodiesterase gene, an oligodendrocytic marker.(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
T Iglesias; S Llanos; M López-Barahona; A Pérez-Aranda; A Rodríguez-Peña; J Bernal; A Höhne; B Seliger; A Muñoz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research     Volume:  5     ISSN:  1044-9523     ISO Abbreviation:  Cell Growth Differ.     Publication Date:  1994 Jul 
Date Detail:
Created Date:  1994-12-07     Completed Date:  1994-12-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9100024     Medline TA:  Cell Growth Differ     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  697-704     Citation Subset:  IM    
Affiliation:
Instituto de Investigaciones Biomédicas, CSIC, Madrid, Spain.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Markers
Brain / cytology,  embryology
Cell Division
Cell Line, Transformed
Cell Transformation, Viral
Fibroblast Growth Factor 2 / pharmacology
Gene Expression Regulation, Neoplastic*
Genes, myc
Insulin / pharmacology
Insulin-Like Growth Factor I / pharmacology
Mice
Neuroglia / metabolism,  pathology*
Oncogene Proteins v-erbA / genetics,  physiology*
Platelet-Derived Growth Factor / pharmacology
Receptors, Thyroid Hormone / genetics,  physiology*
Recombinant Fusion Proteins / metabolism
Stem Cells / metabolism,  pathology*
Triiodothyronine / pharmacology
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Oncogene Proteins v-erbA; 0/Platelet-Derived Growth Factor; 0/Receptors, Thyroid Hormone; 0/Recombinant Fusion Proteins; 103107-01-3/Fibroblast Growth Factor 2; 11061-68-0/Insulin; 67763-96-6/Insulin-Like Growth Factor I; 6893-02-3/Triiodothyronine

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