Document Detail


c-Myc downregulation: a critical molecular event in resveratrol-induced cell cycle arrest and apoptosis of human medulloblastoma cells.
MedLine Citation:
PMID:  16724266     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The correlation of c-Myc expression with resveratrol-induced turnover of medulloblastoma cells was investigated in this study by checking (1) c-Myc expression in medulloblastoma tissues and cell lines (UW228-2 and UW228-3), (2) the in vitro effect of resveratrol on c-Myc expression and (3) the influences of c-Myc inhibition in cell growth and survival. Immunohistochemical staining of human medulloblastomas and noncancerous cerebellar tissues revealed that 8 out of 11 tumor tissues (72.7%) expressed c-Myc, in which 4 cases (50%) showed intensified nuclear labeling. RT-PCR, Western blotting, immunocytochemical and immunofluorescence stainings revealed c-Myc downregulation accompanied with growth suppression and apoptosis. Flow cytometry analysis showed S phase arrest in resveratrol-treated cell populations. Transfection of c-Myc directed antisense oligonucleotides to the cultured medulloblastoma cells could reduce c-Myc expression, inhibit cell growth and arrest the cell cycle at S phase. Our results thus for the first time demonstrate that c-Myc downregulation is a critical molecular event of resveratrol-mediated anti-medulloblastoma activity, which is closely associated with growth suppression, cell cycle arrest and apoptosis of medulloblastoma cells.
Authors:
Peng Zhang; Hong Li; Mo-Li Wu; Xiao-Yan Chen; Qing-You Kong; Xiao-Wei Wang; Yuan Sun; Shu Wen; Jia Liu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-05-25
Journal Detail:
Title:  Journal of neuro-oncology     Volume:  80     ISSN:  0167-594X     ISO Abbreviation:  J. Neurooncol.     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-10-24     Completed Date:  2007-01-04     Revised Date:  2013-06-03    
Medline Journal Info:
Nlm Unique ID:  8309335     Medline TA:  J Neurooncol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  123-31     Citation Subset:  IM    
Affiliation:
Cancer Institute and Liaoning Laboratory of Cancer Genomics, College of Basic Medical Sciences, Dalian Medical University, 116027, Dalian, P. R. China.
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MeSH Terms
Descriptor/Qualifier:
Antioxidants / pharmacology*
Apoptosis / drug effects*
Blotting, Western
Brain Neoplasms / pathology*
Cell Cycle / drug effects*
Cell Line, Tumor
Cell Survival / drug effects
Down-Regulation / drug effects
Flow Cytometry
Fluorescent Antibody Technique
Genes, myc / genetics*
Humans
Immunohistochemistry
Medulloblastoma / pathology*
Neoplasm Proteins / biosynthesis
Oligonucleotides, Antisense / genetics
RNA, Neoplasm / biosynthesis,  genetics
Reverse Transcriptase Polymerase Chain Reaction
S Phase / physiology
Stilbenes / pharmacology*
Transfection
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Neoplasm Proteins; 0/Oligonucleotides, Antisense; 0/RNA, Neoplasm; 0/Stilbenes; Q369O8926L/resveratrol

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