Document Detail


c-Myb is critical for murine colon development.
MedLine Citation:
PMID:  10523863     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The mammalian colon develops from a simple tube of undifferentiated cells into a complex, highly ordered organ, with a continuously self-renewing epithelial layer. We have previously described c-Myb expression in the epithelia of murine and human colon crypts and documented increased expression in colorectal adenocarcinoma cells. To investigate the role of c-Myb in colonic epithelium development, we have used embryos with a disrupted c-myb gene. Prior to the in utero death of these embryos at E15, we excised colon tissue and transplanted it under the kidney capsule of recipient mice to allow further development and cyto-differentiation. Compared to the colons of wildtype and heterozygous littermates, the c-myb homozygous knockout colon is highly irregular with a disordered epithelium and abnormal crypts. In addition, the expression of Bcl-2, a known target of c-Myb, is reduced and apoptosis is increased, indicating a critical requirement for c-Myb in normal colon development.
Authors:
M Zorbas; C Sicurella; I Bertoncello; D Venter; S Ellis; M L Mucenski; R G Ramsay
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Oncogene     Volume:  18     ISSN:  0950-9232     ISO Abbreviation:  Oncogene     Publication Date:  1999 Oct 
Date Detail:
Created Date:  1999-11-19     Completed Date:  1999-11-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8711562     Medline TA:  Oncogene     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  5821-30     Citation Subset:  IM    
Affiliation:
Peter MacCallum Cancer Institute, Melbourne, 8006 Australia.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / physiology
Cell Division / physiology
Cell Movement / physiology
Colon / embryology,  growth & development*,  transplantation,  ultrastructure
Fetus
Gene Expression Regulation, Developmental / physiology
Intestinal Mucosa / embryology,  growth & development,  transplantation,  ultrastructure
Intestine, Small / embryology,  growth & development,  transplantation
Mice
Mice, Congenic
Mice, Inbred C57BL
Mice, Knockout
Proto-Oncogene Proteins c-myb / biosynthesis,  deficiency,  genetics,  physiology*
Chemical
Reg. No./Substance:
0/Proto-Oncogene Proteins c-myb

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