| c-Jun promotes neurite outgrowth and survival in PC12 cells. | |
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MedLine Citation:
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PMID: 11072092 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We investigated the function of c-Jun in PC12 cells by transfecting them with a plasmid containing a c-Jun cDNA transcription cassette. Transfected cells expressed high levels of c-Jun mRNA and protein and demonstrated an increase in both AP-1 DNA binding and gene activation. The c-Jun over-expressing cells showed marked neurite outgrowth but no evidence of spontaneous cell death. In fact, c-Jun over-expressing cells were more resistant to okadaic acid-induced apoptosis. The process outgrowth was not indicative of a full neuronal differentiation response as the transfected PC12 cells did not display action potentials when examined with whole-cell patch-clamping. The phosphorylation of c-Jun on serine 73 appears to be important for this neurite sprouting effect as mutagenesis at this site reduced sprouting whereas a serine 63 mutant tended to increase sprouting. Thus, in PC12 cells c-Jun expression does not induce apoptosis, but rather functions as a neurite outgrowth and neuronal survival signal. |
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Authors:
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M Dragunow; R Xu; M Walton; A Woodgate; P Lawlor; G A MacGibbon; D Young; H Gibbons; J Lipski; A Muravlev; A Pearson; M During |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Brain research. Molecular brain research Volume: 83 ISSN: 0169-328X ISO Abbreviation: Brain Res. Mol. Brain Res. Publication Date: 2000 Nov |
Date Detail:
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Created Date: 2000-12-15 Completed Date: 2001-02-15 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8908640 Medline TA: Brain Res Mol Brain Res Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 20-33 Citation Subset: IM |
Affiliation:
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Department of Molecular Medicine, Faculty of Medicine and Health Science, The University of Auckland, Private Bag 92019, Auckland, New Zealand. m.dragunow@auckland.ac.nz |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Action Potentials
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drug effects,
physiology Animals Apoptosis / drug effects, physiology Cell Differentiation / physiology Cell Survival / physiology Enzyme Inhibitors / pharmacology Genes, Reporter Luciferases / genetics Nerve Growth Factor / pharmacology Neurites / physiology* Neurons / physiology*, ultrastructure* Okadaic Acid / pharmacology PC12 Cells Patch-Clamp Techniques Phenotype Phosphorylation Proto-Oncogene Proteins c-jun / genetics*, metabolism Rats Sodium Channels / physiology Transcription Factor AP-1 / physiology Transfection |
| Chemical | |
Reg. No./Substance:
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0/Enzyme Inhibitors; 0/Proto-Oncogene Proteins c-jun; 0/Sodium Channels; 0/Transcription Factor AP-1; 78111-17-8/Okadaic Acid; 9061-61-4/Nerve Growth Factor; EC 1.13.12.-/Luciferases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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