Document Detail


c-Jun promotes neurite outgrowth and survival in PC12 cells.
MedLine Citation:
PMID:  11072092     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We investigated the function of c-Jun in PC12 cells by transfecting them with a plasmid containing a c-Jun cDNA transcription cassette. Transfected cells expressed high levels of c-Jun mRNA and protein and demonstrated an increase in both AP-1 DNA binding and gene activation. The c-Jun over-expressing cells showed marked neurite outgrowth but no evidence of spontaneous cell death. In fact, c-Jun over-expressing cells were more resistant to okadaic acid-induced apoptosis. The process outgrowth was not indicative of a full neuronal differentiation response as the transfected PC12 cells did not display action potentials when examined with whole-cell patch-clamping. The phosphorylation of c-Jun on serine 73 appears to be important for this neurite sprouting effect as mutagenesis at this site reduced sprouting whereas a serine 63 mutant tended to increase sprouting. Thus, in PC12 cells c-Jun expression does not induce apoptosis, but rather functions as a neurite outgrowth and neuronal survival signal.
Authors:
M Dragunow; R Xu; M Walton; A Woodgate; P Lawlor; G A MacGibbon; D Young; H Gibbons; J Lipski; A Muravlev; A Pearson; M During
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Brain research. Molecular brain research     Volume:  83     ISSN:  0169-328X     ISO Abbreviation:  Brain Res. Mol. Brain Res.     Publication Date:  2000 Nov 
Date Detail:
Created Date:  2000-12-15     Completed Date:  2001-02-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8908640     Medline TA:  Brain Res Mol Brain Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  20-33     Citation Subset:  IM    
Affiliation:
Department of Molecular Medicine, Faculty of Medicine and Health Science, The University of Auckland, Private Bag 92019, Auckland, New Zealand. m.dragunow@auckland.ac.nz
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MeSH Terms
Descriptor/Qualifier:
Action Potentials / drug effects,  physiology
Animals
Apoptosis / drug effects,  physiology
Cell Differentiation / physiology
Cell Survival / physiology
Enzyme Inhibitors / pharmacology
Genes, Reporter
Luciferases / genetics
Nerve Growth Factor / pharmacology
Neurites / physiology*
Neurons / physiology*,  ultrastructure*
Okadaic Acid / pharmacology
PC12 Cells
Patch-Clamp Techniques
Phenotype
Phosphorylation
Proto-Oncogene Proteins c-jun / genetics*,  metabolism
Rats
Sodium Channels / physiology
Transcription Factor AP-1 / physiology
Transfection
Chemical
Reg. No./Substance:
0/Enzyme Inhibitors; 0/Proto-Oncogene Proteins c-jun; 0/Sodium Channels; 0/Transcription Factor AP-1; 78111-17-8/Okadaic Acid; 9061-61-4/Nerve Growth Factor; EC 1.13.12.-/Luciferases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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