Document Detail

c-Jun and c-Fos cooperate with STAT3 in IL-6-induced transactivation of the IL-6 respone element (IRE).
MedLine Citation:
PMID:  11356008     Owner:  NLM     Status:  MEDLINE    
Transcriptional activation of eukaryotic genes often requires the cooperative action of many proteins. The interleukin 6 (IL-6) response element (IRE) is activated by signal transducer and activator of transcription 3 (STAT3), and stimulation with IL-6 leads to STAT3 tyr705 phosphorylation, dimerization, translocation to the nucleus and transactivation of target gene promoters containing IREs. Here, we report that IL-6 and 12-O-tetradecanoylphorbol-13-acetate (TPA) synergistically transactivate the IRE in HepG2 cells, which is coupled to a strong upregulation of c-Jun and c-Fos expression by TPA via the mitogen-activated protein kinase (MAPK) pathway. Overexpression of c-Jun and c-Fos strongly enhanced STAT3-driven IRE transactivation as well as transactivation of the human intercellular adhesion molecule (ICAM)-1 promoter. In contrast, c-Jun mutants lacking the transactivation domain, the DNA-binding domain, or mutants in which the serine residues 63 and 73 were replaced by alanine, did not cooperate with STAT3. In immunoprecipitation experiments, a direct association of STAT3 with c-Jun and c-Fos was observed in response to IL-6. Furthermore, c-Jun/STAT3 and c-Fos/STAT3 complexes were detected on IRE probes in electrophoretic mobility shift assay (EMSA) experiments, but did not bind nor transactivate the TPA response element (TRE). These results demonstrate that activator protein-1 (AP-1) transcription factors can cooperate with STAT3 in IRE transactivation in the absence of direct AP-1 DNA binding.
J J Schuringa; H Timmer; D Luttickhuizen; E Vellenga; W Kruijer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cytokine     Volume:  14     ISSN:  1043-4666     ISO Abbreviation:  Cytokine     Publication Date:  2001 Apr 
Date Detail:
Created Date:  2001-05-17     Completed Date:  2001-08-02     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9005353     Medline TA:  Cytokine     Country:  United States    
Other Details:
Languages:  eng     Pagination:  78-87     Citation Subset:  IM    
Copyright Information:
Copyright 2001 Academic Press.
Department of Genetics, Biological Center, Kerklaan 30, Haren, 9751 NN, The Netherlands.
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MeSH Terms
Blotting, Western
DNA / genetics,  metabolism
DNA-Binding Proteins / metabolism*
Genes, Reporter
Interleukin-6 / genetics*,  pharmacology*
Macromolecular Substances
Precipitin Tests
Protein Binding
Proto-Oncogene Proteins c-fos / genetics,  metabolism*
Proto-Oncogene Proteins c-jun / genetics,  metabolism*
Response Elements / genetics*
STAT3 Transcription Factor
Tetradecanoylphorbol Acetate / pharmacology
Trans-Activators / metabolism*
Transcriptional Activation / drug effects*
Tumor Cells, Cultured
Up-Regulation / drug effects
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Interleukin-6; 0/Macromolecular Substances; 0/Proto-Oncogene Proteins c-fos; 0/Proto-Oncogene Proteins c-jun; 0/STAT3 Transcription Factor; 0/STAT3 protein, human; 0/Trans-Activators; 16561-29-8/Tetradecanoylphorbol Acetate; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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